A thermal expulsion approach to homogeneous large-volume methacrylate monolith preparation; enabling large-scale rapid purification of biomolecules

Danquah, Michael K., Ho, Jenny, & Forde, Gareth M. (2008) A thermal expulsion approach to homogeneous large-volume methacrylate monolith preparation; enabling large-scale rapid purification of biomolecules. Journal of Applied Polymer Science, 109(4), pp. 2426-2433.

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Numerous efforts have been dedicated to the synthesis of large-volume methacrylate monoliths for large-scale biomolecules purification but most were obstructed by the enormous release of exotherms during preparation, thereby introducing structural heterogeneity in the monolith pore system. A significant radial temperature gradient develops along the monolith thickness, reaching a terminal temperature that supersedes the maximum temperature required for structurally homogenous monoliths preparation. The enormous heat build-up is perceived to encompass the heat associated with initiator decomposition and the heat released from free radical-monomer and monomer-monomer interactions. The heat resulting from the initiator decomposition was expelled along with some gaseous fumes before commencing polymerization in a gradual addition fashion. Characteristics of 80 mL monolith prepared using this technique was compared with that of a similar monolith synthesized in a bulk polymerization mode. An extra similarity in the radial temperature profiles was observed for the monolith synthesized via the heat expulsion technique. A maximum radial temperature gradient of only 4.3°C was recorded at the center and 2.1°C at the monolith peripheral for the combined heat expulsion and gradual addition technique. The comparable radial temperature distributions obtained birthed identical pore size distributions at different radial points along the monolith thickness.

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4 citations in Web of Science®

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ID Code: 81552
Item Type: Journal Article
Refereed: Yes
Keywords: Exothermic, Large-volume, Macroporous polymer, Polymerization, Temperature, ABS resins, Biomolecules, Chemical reactions, Concrete pavements, Free radical polymerization, Free radicals, Image segmentation, Monomers, Polymers, Pore size, Purification, Temperature sensors, Thermal gradients, Addition techniques, Bulk polymerizations, Combined heats, Exotherms, Initiator decompositions, Maximum temperatures, Methacrylate monoliths, Pore size distributions, Pore systems, Radial temperature distributions, Radial temperature gradients, Radial temperature profiles, Rapid purifications, Structural heterogeneities, Synthesis of, Terminal temperatures, Monolithic integrated circuits, monomer, synthesis, temperature effect, thermal property
DOI: 10.1002/app.28346
ISSN: 0021-8995
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 05 Feb 2015 23:06
Last Modified: 11 Feb 2015 04:26

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