Performance of R-N(R′)-R″ functionalised poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic sorbent for plasmid DNA adsorption

Danquah, Michael K., Ho, Jenny, & Forde, Gareth M. (2007) Performance of R-N(R′)-R″ functionalised poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic sorbent for plasmid DNA adsorption. Journal of Separation Science, 30(17), pp. 2843-2850.

View at publisher


High-throughput plasmid DNA (pDNA) manufacture is obstructed predominantly by the performance of conventional stationary phases. For this reason, the search for new materials for fast chromatographic separation of pDNA is ongoing. A poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (GMA-EGDMA) monolithic material was synthesised via a thermal-free radical reaction, functionalised with different amino groups from urea, 2-chloro-N,N-diethylethylamine hydrochloride (DEAE-Cl) and ammonia in order to investigate their plasmid adsorption capacities. Physical characterisation of the monolithic polymer showed a macroporous polymer having a unimodal pore size distribution pivoted at 600 nm. Chromatographic characterisation of the functionalised polymers using pUC19 plasmid isolated from E. coli DH5α-pUC19 showed a maximum plasmid adsorption capacity of 18.73 mg pDNA/mL with a dissociation constant (KD) of 0.11 mg/mL for GMA-EGDMA/DEAE-Cl polymer. Studies on ligand leaching and degradation demonstrated the stability of GMA-EGDMA/DEAE-Cl after the functionalised polymers were contacted with 1.0 M NaOH, which is a model reagent for most 'cleaning in place' (CIP) systems. However, it is the economic advantage of an adsorbent material that makes it so attractive for commercial purification purposes. Economic evaluation of the performance of the functionalised polymers on the grounds of polymer cost (PC)/mg pDNA retained endorsed the suitability of GMA-EGDMA/DEAE-Cl polymer.

Impact and interest:

11 citations in Scopus
Search Google Scholar™
10 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 81564
Item Type: Journal Article
Refereed: Yes
Keywords: Adsorption capacity, Chromatography, Economics, Methacrylate monolith, Plasmid DNA, Cleaning in place (CIP), Poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate), Adsorption, DNA, Economic analysis, Free radical reactions, Ligands, Polyethylene glycols, Sorbents, Synthesis (chemical), Functional polymers, 2 chloro n, n diethylethylamine, amine, ammonia, copolymer, poly(glycidyl methacrylate co ethylene glycol dimethacrylate), sorbent, unclassified drug, urea, article, chemical reaction, degradation, dissociation constant, economic evaluation, leaching, porosity, priority journal, separation technique, synthesis, Chromatography, High Pressure Liquid, DNA, Bacterial, Escherichia coli, Methacrylates, Molecular Structure, Particle Size, Plasmids, Reproducibility of Results, Sensitivity and Specificity, Surface Properties, Time Factors
DOI: 10.1002/jssc.200700217
ISSN: 1615-9306
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 05 Feb 2015 23:34
Last Modified: 11 Feb 2015 05:21

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page