Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein

Forde, Gareth M. (2008) Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein. In Zachariou, Michael (Ed.) Affinity Chromatography: Methods and Protocols [2nd ed.]. Humana Press (Springer), Totowa, NJ, pp. 125-136.

View at publisher


Methods are presented for the preparation, ligand density analysis and use of an affinity adsorbent for the purification of a glutathione S-transferase (GST) fusion protein in packed and expanded bed chromatographic processes. The protein is composed of GST fused to a zinc finger transcription factor (ZnF). Glutathione, the affinity ligand for GST purification, is covalently immobilized to a solid-phase adsorbent (Streamline™). The GST–ZnF fusion protein displays a dissociation constant of 0.6 x10-6 M to glutathione immobilized to Streamline™. Ligand density optimization, fusion protein elution conditions (pH and glutathione concentration) and ligand orientation are briefly discussed.

Impact and interest:

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 81617
Item Type: Book Chapter
Additional URLs:
Keywords: GST fusion protein, affinity purification, chromatography, expanded bed adsorption
DOI: 10.1007/978-1-59745-582-4_9
ISBN: 9781588296597
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 09 Feb 2015 23:21
Last Modified: 13 Feb 2015 04:31

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page