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Independent expression of Rep and RepA and their roles in regulating bean yellow dwarf virus replication

Hefferon, Kathleen L. & Dugdale, Benjamin (2003) Independent expression of Rep and RepA and their roles in regulating bean yellow dwarf virus replication. Journal of General Virology, 84(12), pp. 3465-3472.

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Abstract

Bean yellow dwarf virus (BeYDV) is a mastrevirus specific for dicotyledenous hosts. It contains four ORFs encoding a movement protein, a coat protein, and two Rep gene products, Rep and RepA, which are encoded by two overlapping ORFs. In this study, the roles of Rep and RepA in regulating replication of the BeYDV-based replicon were investigated by uncoupling them and placing Rep and RepA each under constitutive promoter control. Constitutive expression of both Rep and RepA supported replication and enhanced gene expression. When a reporter plasmid containing the Rep gene in the context of its native promoter was supplemented with additional Rep protein, replication was enhanced but the increase in gene expression was found to be more modest. Furthermore, expression of constitutively expressed RepA alone was found to reduce replication of this reporter construct as well as delay BeYDV replication in general. The effect of a RepA mutant with an altered retinoblastoma-related-protein binding motif on the efficiency of BeYDV replication was also examined. This mutant was found to severely diminish replication efficiency. Finally, the relationship of BeYDV coat protein to virus replication and reporter gene expression was investigated. Addition of coat protein increased accumulation of single-stranded DNA and had a detrimental effect on reporter gene expression.

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14 citations in Scopus
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12 citations in Web of Science®

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ID Code: 8183
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author. Author contact details: klh22@cornell.edu
Keywords: BeYDV mastrevirus
DOI: 10.1099/vir.0.19494-0
ISSN: 0022-1317
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Virology (060506)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2003 Society for General Microbiology
Deposited On: 20 Jun 2007
Last Modified: 29 Feb 2012 23:30

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