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An iterated sequence in the genome of banana bunchy top virus is essential for efficient replication

Herrera-Valencia, Virginia A., Dugdale, Benjamin, Harding, Robert M., & Dale, James L. (2006) An iterated sequence in the genome of banana bunchy top virus is essential for efficient replication. Journal of General Virology, 87, pp. 3409-3412.

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Abstract

Banana bunchy top virus (BBTV), a multi-component circular ssDNA virus, replicates via a dsDNA intermediate that also serves as a template for virion sense transcription. Seven virus-derived transcripts have been previously identified and analysed in BBTV-infected bananas by northern analysis and 3' rapid amplification of cDNA ends (3' RACE). In this study, we have used RNA ligase-mediated rapid amplification of 5' cDNA ends (RLM-RACE) to complete the mapping of the BBTV gene transcripts and have now fully mapped the transcribed regions of each BBTV component and effectively defined the upstream regulatory region.

Impact and interest:

10 citations in Scopus
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8 citations in Web of Science®

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ID Code: 8198
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author. Author contact details: r.harding@qut.edu.au
DOI: 10.1099/vir.0.82166-0
ISSN: 1465-2099
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2006 The Society of General Microbiology
Deposited On: 21 Jun 2007
Last Modified: 29 Feb 2012 23:27

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