Taro vein chlorosis virus: Characterisation and variability of a new nucleorhabdovirus

Revill, Peter, Trinh, Xuan, Dale, James L., & Harding, Robert M. (2005) Taro vein chlorosis virus: Characterisation and variability of a new nucleorhabdovirus. Journal of General Virology, 86, pp. 491-499.

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Abstract

We have sequenced the monopartite RNA genome of a Fijian isolate of Taro vein chlorosis virus (TaVCV) and confirmed that it is a definitive rhabdovirus with most similarity to members of the genus Nucleorhabdovirus. The 12020 nt TaVCV negative-sense RNA genome contained six ORFs in the antigenomic sequence, equivalent to genes N, P, 3, M, G and L identified in other rhabdoviruses. The putative gene products had closest identity to those of the nucleorhabdovirus, Maize mosaic virus (MMV). A characteristic 3'AAUUCUUUUUGGGUUGU/A 5' sequence was identified in each of the intergenic regions, and the TaVCV leader and trailer sequences comprised 140 nt and 61 nt, respectively. Assignment of TaVCV into the genus Nucleorhabdovirus was supported by thin-section electron microscopy of TaVCV-infected taro leaves, which identified virions budding from nuclear membranes into the perinuclear space. Variability studies identified high levels of TaVCV sequence diversity. Within the L gene of 20 TaVCV isolates from Fiji, Federated States of Micronesia, New Caledonia, Papua New Guinea, Solomon Islands and Vanuatu, maximum variability at the nucleotide level was 27.4%. Within the N gene, maximum variability among 15 isolates at the nucleotide level was 19.3%. The high level of TaVCV variability observed suggested that the introduction of TaVCV to the Pacific Islands is not a recent occurrence.

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ID Code: 8202
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal’s website (see hypertext link) or contact the author.
Author contact details: r.harding@qut.edu.au
DOI: 10.1099/vir.0.80591-0
ISSN: 0022-1317
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2005 Society for General Microbiology
Deposited On: 21 Jun 2007 00:00
Last Modified: 29 Feb 2012 13:13

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