Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells
Elliott, A.R., Campbell, J.A., Dugdale, B., Brettell, R.I.S., & Grof, C.P.L. (1999) Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells. Plant Cell Reports, 18(9), pp. 707-714.
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Early detection of plant transformation events is necessary for the rapid establishment and optimization of plant transformation protocols. We have assessed modified versions of the green fluorescent protein (GFP) from Aequorea victoria as early reporters of plant transformation using a dissecting fluorescence microscope with appropriate filters. Gfp-expressing cells from four different plant species (sugarcane, maize, lettuce, and tobacco) were readily distinguished, following either Agrobacterium-mediated or particle bombardment-mediated transformation. The identification of gfp-expressing sugarcane cells allowed for the elimination of a high proportion of non-expressing explants and also enabled visual selection of dividing transgenic cells, an early step in the generation of transgenic organisms. The recovery of transgenic cell clusters was streamlined by the ability to visualize gfp-expressing tissues in vitro.
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|Item Type:||Journal Article|
|Keywords:||green fluorescent protein, sugarcane, transformation, Agrobacterium|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 1999 Springer|
|Deposited On:||08 Mar 2015 23:31|
|Last Modified:||08 Mar 2015 23:31|
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