Optimising ketocarotenoid production in potato tubers : effect of genetic background, transgene combinations and environment

Campbell, Raymond, Morris, Wayne L., Mortimer, Cara L., Misawa, Norihiko, Ducreux, Laurence J.M., Morris, Jenny A., Hedley, Pete E., Fraser, Paul D., & Taylor, Mark A. (2015) Optimising ketocarotenoid production in potato tubers : effect of genetic background, transgene combinations and environment. Plant Science, 234, pp. 27-37.

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Astaxanthin is a high value carotenoid produced by some bacteria, a few green algae, several fungi but only a limited number of plants from the genus Adonis. Astaxanthin has been industrially exploited as a feed supplement in poultry farming and aquaculture. Consumption of ketocarotenoids, most notably astaxanthin, is also increasingly associated with a wide range of health benefits,as demonstrated in numerous clinical studies. Currently astaxanthin is produced commercially by chemical synthesis or from algal production systems. Several studies have used a metabolic engineering approach to produce astaxanthin in transgenic plants. Previous attempts to produce transgenic potato tubers biofortified with astaxanthin have met with limited success. In this study we have investigated approaches to optimising tuber astaxanthin content. It is demonstrated that the selection of appropriate parental genotype for transgenic approaches and stacking carotenoid biosynthetic pathway genes with the cauliflower Or gene result in enhanced astaxanthin content, to give six-fold higher tuber astaxanthin content than has been achieved previously. Additionally we demonstrate the effects of growth environment on tuber carotenoid content in both wild type and astaxanthin-producing transgenic lines and describe the associated transcriptome and metabolome restructuring.

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ID Code: 82353
Item Type: Journal Article
Refereed: Yes
Keywords: Astaxanthin; Carotenoid; Environment; Ketocarotenoid; Microarray; Potato
DOI: 10.1016/j.plantsci.2015.01.014
ISSN: 0168-9452
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2015 Elsevier
Copyright Statement: This is the author’s version of a work that was accepted for publication in Plant Science. Changes resulting from the publishing process, such as peer review, editing,corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Plant Science, Volume 234, May 2015, Pages 27–37 DOI: 10.1016/j.plantsci.2015.01.014
Deposited On: 11 Mar 2015 00:13
Last Modified: 15 Mar 2015 04:57

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