Evaluation of intra- and extra-epithelial secretory IgA in chlamydial infections
Armitage, Charles W., O'Meara, Connor P., Harvie, Marina C.G., Timms, Peter, Wijburg, Odilia L., & Beagley, Kenneth W. (2014) Evaluation of intra- and extra-epithelial secretory IgA in chlamydial infections. Immunology, 143(4), pp. 520-530.
IgA is an important mucosal antibody that can neutralize mucosal pathogens by either preventing attachment to epithelia (immune exclusion) or alternatively inhibit intraepithelial replication following transcytosis by the polymeric immunoglobulin receptor (pIgR). Chlamydia trachomatis is a major human pathogen that initially targets the endocervical or urethral epithelium in women and men, respectively. As both tissues contain abundant SIgA we assessed the protection afforded by IgA targeting different chlamydial antigens expressed during the extra and intraepithelial stages of infection. We developed an in vitro model utilizing polarizing cells expressing the murine pIgR together with antigen-specific mouse IgA, and an in vivo model utilizing pIgR-/- mice. SIgA targeting the extraepithelial chlamydial antigen, the major outer membrane protein (MOMP), significantly reduced infection in vitro by 24 % and in vivo by 44 %. Conversely, pIgR-mediated delivery of IgA targeting the intraepithelial inclusion membrane protein A (IncA) bound to the inclusion but did not reduce infection in vitro or in vivo. Similarly, intraepithelial IgA targeting the secreted protease Chlamydia protease-like activity factor (CPAF) also failed to reduce infection. Together, these data suggest the importance of pIgR-mediated delivery of IgA targeting extra but not intraepithelial chlamydial antigens for protection against a genital tract infection.
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|Item Type:||Journal Article|
|Keywords:||Immunology, Chlamydia, IgA, polymeric Ig receptor, vaccine|
|Divisions:||Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Copyright Owner:||Copyright 2014 John Wiley & Sons Ltd|
|Copyright Statement:||This is the accepted version of the following article: Armitage, C. W., O'Meara, C. P., Harvie, M. C. G., Timms, P., Wijburg, O. L. and Beagley, K. W. (2014), Evaluation of intra- and extra-epithelial secretory IgA in chlamydial infections. Immunology, 143: 520–530, which has been published in final form at doi: 10.1111/imm.12317|
|Deposited On:||19 Mar 2015 23:10|
|Last Modified:||04 Jan 2016 15:41|
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