Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen
Hooper, John D., Zijlstra, Andries, Liang, Hongyan, Claassen, Gisela F., Tarin, David, Testa, Jacqueline E., Quigley, James P., & Aimes, Ronald T. (2003) Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen. Oncogene, 22(12), pp. 1783-1794.
We have previously used a subtractive immunization (SI) approach to generate monoclonal antibodies (mAbs) against proteins preferentially expressed by the highly metastatic human epidermoid carcinoma cell line, M+HEp3. Here we report the immunopurication, identication and characterization of SIMA135/CDCP1 (subtractive immunization M+HEp3 associated 135 kDa protein/CUB domain containing protein 1) using one of these mAbs designated 41-2.
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|Item Type:||Journal Article|
|Additional Information:||For more information, please refer to the journal's website (see hypertext link) or contact the author. Author contact details: email@example.com|
|Keywords:||subtractive immunization, metastasis, SIMA135/CDCP1, proteomics, colon cancer, CUB|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)|
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2003 Nature Publishing Group|
|Deposited On:||26 Jun 2007|
|Last Modified:||29 Feb 2012 23:20|
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