Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen
Hooper, John D., Zijlstra, Andries, Liang, Hongyan, Claassen, Gisela F., Tarin, David, Testa, Jacqueline E., Quigley, James P., & Aimes, Ronald T. (2003) Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen. Oncogene, 22(12), pp. 1783-1794.
We have previously used a subtractive immunization (SI) approach to generate monoclonal antibodies (mAbs) against proteins preferentially expressed by the highly metastatic human epidermoid carcinoma cell line, M+HEp3. Here we report the immunopurication, identication and characterization of SIMA135/CDCP1 (subtractive immunization M+HEp3 associated 135 kDa protein/CUB domain containing protein 1) using one of these mAbs designated 41-2.
Impact and interest:
Citation counts are sourced monthly from and citation databases.
These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.
Citations counts from theindexing service can be viewed at the linked Google Scholar™ search.
|Item Type:||Journal Article|
|Additional Information:||For more information, please refer to the journal's website (see hypertext link) or contact the author.
Author contact details: firstname.lastname@example.org
|Keywords:||subtractive immunization, metastasis, SIMA135/CDCP1, proteomics, colon cancer, CUB|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2003 Nature Publishing Group|
|Deposited On:||26 Jun 2007 00:00|
|Last Modified:||29 Feb 2012 13:20|
Repository Staff Only: item control page