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Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen

Hooper, John D., Zijlstra, Andries, Liang, Hongyan, Claassen, Gisela F., Tarin, David, Testa, Jacqueline E., Quigley, James P., & Aimes, Ronald T. (2003) Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135kDa cell surface phosphorylated glycoprotein antigen. Oncogene, 22(12), pp. 1783-1794.

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Abstract

We have previously used a subtractive immunization (SI) approach to generate monoclonal antibodies (mAbs) against proteins preferentially expressed by the highly metastatic human epidermoid carcinoma cell line, M+HEp3. Here we report the immunopuri﬿cation, identi﬿cation and characterization of SIMA135/CDCP1 (subtractive immunization M+HEp3 associated 135 kDa protein/CUB domain containing protein 1) using one of these mAbs designated 41-2.

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ID Code: 8263
Item Type: Journal Article
Additional Information: For more information, please refer to the journal's website (see hypertext link) or contact the author. Author contact details: jd.hooper@qut.edu.au
Keywords: subtractive immunization, metastasis, SIMA135/CDCP1, proteomics, colon cancer, CUB
DOI: 10.1038/sj.onc.1206220
ISSN: 0950-9232
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2003 Nature Publishing Group
Deposited On: 26 Jun 2007
Last Modified: 29 Feb 2012 23:20

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