Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization

Tainaka, Kazuki, Kubota, Shimpei I, Suyama, Takeru Q, Susaki, Etsuo A, Perrin, Dimitri, Ukai-Tadenuma, Maki, Ukai, Hideki, & Ueda, Hiroki R (2014) Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization. Cell, 159(4), pp. 911-924.

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The development of whole-body imaging at single-cell resolution enables system-level approaches to studying cellular circuits in organisms. Previous clearing methods focused on homogenizing mismatched refractive indices of individual tissues, enabling reductions in opacity but falling short of achieving transparency. Here, we show that an aminoalcohol decolorizes blood by efficiently eluting the heme chromophore from hemoglobin. Direct transcardial perfusion of an aminoalcohol-containing cocktail that we previously termed CUBIC coupled with a 10 day to 2 week clearing protocol decolorized and rendered nearly transparent almost all organs of adult mice as well as the entire body of infant and adult mice. This CUBIC-perfusion protocol enables rapid whole-body and whole-organ imaging at single-cell resolution by using light-sheet fluorescent microscopy. The CUBIC protocol is also applicable to 3D pathology, anatomy, and immunohistochemistry of various organs. These results suggest that whole-body imaging of colorless tissues at high resolution will contribute to organism-level systems biology.

Impact and interest:

68 citations in Scopus
68 citations in Web of Science®
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ID Code: 82687
Item Type: Journal Article
Refereed: Yes
Additional URLs:
DOI: 10.1016/j.cell.2014.10.034
ISSN: 0092-8674
Divisions: Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Cell Press / Elsevier
Deposited On: 24 Mar 2015 03:04
Last Modified: 26 Jun 2017 10:54

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