A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction

Zijlstra, Andries, Mellor, Rebecca, Panzarella, Giano, Aimes, Ronald T., Hooper, John D., Marchenko, Natalia D., & Quigley, James P. (2002) A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction. Cancer Research, 62(23), pp. 7083-7092.

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Abstract

A quantitative assessment of rate-limiting steps in metastasis has always been challenging because of the difficulty of detecting small tumor cell populations. We have developed a highly sensitive assay for monitoring the metastatic dissemination of human tumor cells in the chick embryo and used this assay to investigate the relative efficacy of sequential stages in the metastatic cascade for two malignant human tumor cells lines, HEp3 and HT1080. This assay is based on the real-time PCR amplification of human alu sequences and exhibits a high sensitivity (25 cells/lung) with a large linear range (50–100,000 cell/lung). The assay is optimized for a high number of replicate in vivo assays (50–100 animals/assay) and can be applied in both experimental and spontaneous metastasis models.

Impact and interest:

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ID Code: 8275
Item Type: Journal Article
Additional Information: Articles free to read on journal website after 12 months
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ISSN: 0008-5472
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Copyright Owner: Copyright 2002 American Association for Cancer Research
Deposited On: 27 Jun 2007
Last Modified: 28 Jan 2015 02:01

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