Functional and mechanistic investigation of Shikonin in scarring

Xie, Yan, Fan, Chen, Dong, Ying, Lynam, Emily, Leavesley, David I., Li, Kun, Su, Yonghua, Yang, Yinxue, & Upton, Zee (2015) Functional and mechanistic investigation of Shikonin in scarring. Chemico-Biological Interactions, 228, pp. 18-27.

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Abstract

Scarring is a significant medical burden; financially to the health care system and physically and psychologically for patients. Importantly, there have been numerous case reports describing the occurrence of cancer in burn scars. Currently available therapies are not satisfactory due to their undesirable side-effects, complex delivery routes, requirements for long-term use and/or expense. Radix Arnebiae (Zi Cao), a perennial herb, has been clinically applied to treat burns and manage scars for thousands of years in Asia. Shikonin, an active component extracted from Radix Arnebiae, has been demonstrated to induce apoptosis in cancer cells. Apoptosis is an essential process during scar tissue remodelling. It was therefore hypothesized that Shikonin may induce apoptosis in scar-associated cells. This investigation presents the first detailed in vitro study examining the functional responses of scar-associated cells to Shikonin, and investigates the mechanisms underlying these responses. The data obtained suggests that Shikonin inhibits cell viability and proliferation and reduces detectable collagen in scar-derived fibroblasts. Further investigation revealed that Shikonin induces apoptosis in scar fibroblasts by differentially regulating the expression of caspase 3, Bcl-2, phospho-Erk1/2 and phospho-p38. In addition, Shikonin down-regulates the expression of collagen I, collagen III and alpha-smooth muscle actin genes hence attenuating collagen synthesis in scar-derived fibroblasts. In summary, it is demonstrated that Shikonin induces apoptosis and decreases collagen production in scar-associated fibroblasts and may therefore hold potential as a novel scar remediation therapy.

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ID Code: 82778
Item Type: Journal Article
Refereed: Yes
Additional Information: Kun Li not found on QUT Virtual
Keywords: Scar remediation therapy, Apoptosis, Collagen reduction, Shikonin
DOI: 10.1016/j.cbi.2014.12.037
ISSN: 00092797
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2015 Elsevier B.V.
Deposited On: 25 Mar 2015 22:36
Last Modified: 27 Mar 2015 00:33

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