Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides

Kenyon, Johanna J., Holt, Kathryn E., Pickard, Derek, Dougan, Gordon, & Hall, Ruth M. (2014) Insertions in the OCL1 locus of Acinetobacter baumannii lead to shortened lipooligosaccharides. Research in Microbiology, 165(6), pp. 472-475.

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Abstract

Genomes of 82 Acinetobacter baumannii global clones 1 (GC1) and 2 (GC2) isolates were sequenced and different forms of the locus predicted to direct synthesis of the outer core (OC) of the lipooligosaccharide were identified. OCL1 was in all GC2 genomes, whereas GC1 isolates carried OCL1, OCL3 or a new locus, OCL5. Three mutants in which an insertion sequence (ISAba1 or ISAba23) interrupted OCL1 were identified. Isolates with OCL1 intact produced only lipooligosaccharide, while the mutants produced lipooligosaccharide of reduced molecular weight. Thus, the assignment of the OC locus as that responsible for the synthesis of the OC is correct.

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ID Code: 83184
Item Type: Journal Article
Refereed: Yes
Keywords: Acinetobacter baumannii, OC locus, lipooligosaccharide, polysaccharide, Genetic variation, Genome, Sequence, Clone, bacterial pathogen, hospital-acquired infection, O-antigen
DOI: 10.1016/j.resmic.2014.05.034
ISSN: 0923-2508
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > GENETICS (060400) > Genomics (060408)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Bacteriology (060501)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Microbial Genetics (060503)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Funding:
Copyright Owner: Copyright 2015 Elsevier B.V.
Deposited On: 02 Dec 2015 02:17
Last Modified: 08 Dec 2015 03:57

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