Meltdown : a tool to help in the interpretation of thermal melt curves acquired by differential scanning fluorimetry

Rosa, N., Ristic, M., Seabrook, S.A., Lovell, D.R., Lucent, D., & Newman, J. (2015) Meltdown : a tool to help in the interpretation of thermal melt curves acquired by differential scanning fluorimetry. Journal of Biomolecular Screening, 20(7), pp. 898-905.

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The output of a differential scanning fluorimetry (DSF) assay is a series of melt curves, which need to be interpreted to get value from the assay. An application that translates raw thermal melt curve data into more easily assimilated knowledge is described. This program, called “Meltdown,” conducts four main activities—control checks, curve normalization, outlier rejection, and melt temperature (Tm) estimation—and performs optimally in the presence of triplicate (or higher) sample data. The final output is a report that summarizes the results of a DSF experiment. The goal of Meltdown is not to replace human analysis of the raw fluorescence data but to provide a meaningful and comprehensive interpretation of the data to make this useful experimental technique accessible to inexperienced users, as well as providing a starting point for detailed analyses by more experienced users.

Impact and interest:

7 citations in Scopus
7 citations in Web of Science®
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ID Code: 84017
Item Type: Journal Article
Refereed: Yes
Keywords: differential scanning fluorimetry, thermal shift analysis, high-throughput formulation screening, Thermofluor
DOI: 10.1177/1087057115584059
ISSN: 1552-454X
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > ANALYTICAL CHEMISTRY (030100)
Australian and New Zealand Standard Research Classification > INFORMATION AND COMPUTING SCIENCES (080000) > COMPUTER SOFTWARE (080300)
Divisions: Current > Schools > School of Electrical Engineering & Computer Science
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2015 Society for Laboratory Automation and Screening
Deposited On: 11 May 2015 01:24
Last Modified: 17 Mar 2016 10:10

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