Four generations of sequencing: Is it ready for the clinic yet?

Srinivasan, Srilakshmi & Batra, Jyotsna (2014) Four generations of sequencing: Is it ready for the clinic yet? Journal of Next Generation Sequencing & Applications, 1(107).

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Abstract

Next-generation sequencing techniques have revolutionized over the last decade providing researchers with low cost, high-throughput alternatives compared to the traditional Sanger sequencing methods. These sequencing techniques have rapidly evolved from first-generation to fourth-generation with very broad applications such as unravelling the complexity of the genome, in terms of genetic variations, and having a high impact on the biological field. In this review, we discuss the transition of sequencing from the second-generation to the third- and fourth-generations, and describe some of their novel biological applications. With the advancement in technology, the earlier challenges of minimal size of the instrument, flexibility of throughput, ease of data analysis and short run times are being addressed. However, the need for prospective analysis and effectiveness to test whether the knowledge of any given new variants identified has an effect on clinical outcome may need improvement.

Impact and interest:

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ID Code: 84216
Item Type: Review
Refereed: Yes
Keywords: NGS, TGS, whole genome sequencing, amplification, read-length
DOI: 10.4172/jngsa.1000107
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 The Author(s)
Deposited On: 19 May 2015 01:48
Last Modified: 19 May 2015 01:48

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