Failure to launch: The self-regulating Md -MYB10R6 gene from apple is active in flowers but not leaves of Petunia

Boase, Murray R., Brendolise, Cyril, Wang, Lei, Ngo, Hahn, Espley, Richard V., Hellens, Roger P., Schwinn, Kathy E., Davies, Kevin M., & Albert, Nick W. (2015) Failure to launch: The self-regulating Md -MYB10R6 gene from apple is active in flowers but not leaves of Petunia. Plant Cell Reports, 34(10), pp. 1817-1823.

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Abstract

Coloured foliage due to anthocyanin pigments (bronze/red/black) is an attractive trait that is often lacking in many bedding, ornamental and horticultural plants. Apples (Malus × domestica) containing an allelic variant of the anthocyanin regulator, Md-MYB10R6, are highly pigmented throughout the plant, due to autoregulation by MYB10 upon its own promoter. We investigated whether Md-MYB10R6 from apple is capable of functioning within the heterologous host Petunia hybrida to generate plants with novel pigmentation patterns. The Md-MYB10R6 transgene (MYB10–R6pro:MYB10:MYB10term) activated anthocyanin synthesis when transiently expressed in Antirrhinumroseadorsea petals and petunia leaf discs. Stable transgenic petunias containing Md-MYB10R6 lacked foliar pigmentation but had coloured flowers, complementing the an2 phenotype of ‘Mitchell’ petunia. The absence of foliar pigmentation was due to the failure of the Md-MYB10R6 gene to self-activate in vegetative tissues, suggesting that additional protein partners are required for Md-MYB10 to activate target genes in this heterologous system. In petunia flowers, where endogenous components including MYB-bHLH-WDR (MBW) proteins were present, expression of the Md-MYB10R6 promoter was initiated, allowing auto-regulation to occur and activating anthocyanin production. Md-MYB10 is capable of operating within the petunia MBW gene regulation network that controls the expression of the anthocyanin biosynthesis genes, AN1 (bHLH) and MYBx (R3-MYB repressor) in petals.

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2 citations in Scopus
2 citations in Web of Science®
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ID Code: 86189
Item Type: Journal Article
Refereed: Yes
Keywords: Anthocyanin, Flavonoid, MYB, BHLH, WDR, Petunia
DOI: 10.1007/s00299-015-1827-4
ISSN: 0721-7714
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2015 Springer Berlin Heidelberg
Deposited On: 04 Aug 2015 22:54
Last Modified: 19 May 2016 04:01

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