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Human adipose tissue endothelial cells promote preadipocyte proliferation

Hutley, Louise J., Herington, Adrian C., Shurety, Wenda, Cheung, Catherine, Vesey, David A., Cameron, Donald P., & Prins, John B. (2001) Human adipose tissue endothelial cells promote preadipocyte proliferation. American Journal of Physiology - Endocrinology and Metabolism, 281(5), E1037-E1044.

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Abstract

Adipogenesis is preceded by development of a microvascular network, and optimal functioning of adipose tissue as an energy store and endocrine organ is dependent on extensive vascularization. We have examined the role of endothelial cell-derived factors that influence the proliferation of human preadipocytes. Microvascular endothelial cells and preadipocytes were isolated from human omental and subcutaneous adipose tissue biopsies by use of a developed procedure of collagenase digest, immunoselection, and differential trypsinization. Conditioned medium from microvascular endothelial cell cultures promoted the proliferation of preadipocytes (P = <0.001) and (to a lesser extent) other cell types. No depot-specific differences in mitogenic capacity of microvascular endothelial cell medium or of preadipocyte response were observed. These results indicate that adipose tissue endothelial cells secrete soluble adipogenic factor(s).

Impact and interest:

83 citations in Scopus
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74 citations in Web of Science®

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ID Code: 8725
Item Type: Journal Article
Additional Information: Articles free to read on journal website after 12 months
Keywords: adipose tissue, preadipocyte differentiation, endothelial cells, FGF, 1
ISSN: 0193-1849
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300) > Endocrinology (110306)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2001 American Physiological Society
Deposited On: 23 Jul 2007
Last Modified: 22 Jan 2015 05:11

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