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Human adipose tissue endothelial cells promote preadipocyte proliferation

Hutley, Louise J., Herington, Adrian C., Shurety, Wenda, Cheung, Catherine, Vesey, David A., Cameron, Donald P., & Prins, John B. (2001) Human adipose tissue endothelial cells promote preadipocyte proliferation. American Journal of Physiology - Endocrinology and Metabolism, 281(5), E1037-E1044.


Adipogenesis is preceded by development of a microvascular network, and optimal functioning of adipose tissue as an energy store and endocrine organ is dependent on extensive vascularization. We have examined the role of endothelial cell-derived factors that influence the proliferation of human preadipocytes. Microvascular endothelial cells and preadipocytes were isolated from human omental and subcutaneous adipose tissue biopsies by use of a developed procedure of collagenase digest, immunoselection, and differential trypsinization. Conditioned medium from microvascular endothelial cell cultures promoted the proliferation of preadipocytes (P = <0.001) and (to a lesser extent) other cell types. No depot-specific differences in mitogenic capacity of microvascular endothelial cell medium or of preadipocyte response were observed. These results indicate that adipose tissue endothelial cells secrete soluble adipogenic factor(s).

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ID Code: 8725
Item Type: Journal Article
Additional Information: For more information, please refer to the journal’s website (see hypertext link) or contact the author. Author contact details: a.herington@qut.edu.au
Additional URLs:
Keywords: adipose tissue, preadipocyte differentiation, endothelial cells, FGF, 1
ISSN: 0193-1849
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300) > Endocrinology (110306)
Divisions: Past > QUT Faculties & Divisions > Faculty of Science and Technology
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2001 American Physiological Society
Deposited On: 23 Jul 2007
Last Modified: 10 Aug 2011 23:27

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