Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis
Kong, Hee Jeong, Lee, Ye-Ji, Park, In-Suk, Lee, Won Woo, Kim, Young-Ok, Nam, Bo-Hye, Kim, Woo-Jin, Jung, Hyungtaek, Jeon, You-Jin, An, Cheul Min, & Lee, Sang-Jun (2013) Molecular and functional characterizations of a Kunitz-type serine protease inhibitor FcKuSPI of the shrimp Fenneropenaeus chinensis. Fish and Shellfish Immunology, 35(3), pp. 1025-1029.
Serine proteinase inhibitors play important and diverse roles in biological processes such as coagulation, defense mechanisms, and immune responses. Here, we identified and characterized a Kunitz-type proteinase inhibitor, designated FcKuSPI, of the BPTI/Kunitz family of serine proteinase inhibitors from the hemocyte cDNA library of the shrimp Fenneropenaeus chinensis. The deduced amino acid sequence of FcKuSPI comprises 80 residues with a putative signal peptide of 15 amino acids. The predicted molecular weight of the mature peptide is 7.66 kDa and its predicted isoelectric point is 8.84. FcKuSPI includes a Kunitz domain containing six conserved cysteine residues that are predicted to form three disulfide bonds. FcKuSPI shares 44e53% homology with BPTI/Kunitz family members from other species. FcKuSPI mRNAwas expressed highly in the hemocytes and moderately in muscle in healthy shrimp. Recombinant FcKuSPI protein demonstrated anti-protease activity against trypsin and anticoagulant activity against citrated human plasma in a dose-dependent manner in in vitro assays.
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|Item Type:||Journal Article|
|Keywords:||Kunitz-type, Serine proteinase inhibitor, Fenneropenaeus chinensis, Hemocyte, Anticoagulant|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2013 Elsevier|
|Deposited On:||09 Sep 2015 23:07|
|Last Modified:||14 Sep 2015 21:57|
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