Characterization of novel microsatellite markers derived from Korean rose bitterling (Rhodeus uyekii) genomic library

Kim, W.J., Shin, E.H., Kong, H.J., Kim, H.S., Kim, B.S., Nam, B.H., Kim, Y.O., Kim, C.H., Jung, H., & An, C.M. (2014) Characterization of novel microsatellite markers derived from Korean rose bitterling (Rhodeus uyekii) genomic library. Genetics and Molecular Research, 13(4), pp. 8147-8152.

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Abstract

Korean rose bitterling (Rhodeus uyekii) is a freshwater fish endemic to Korea. Natural populations of this species have experienced severe declines as a result of habitat fragmentation and water pollution. To conserve and restore R. uyekii, the genetic diversity of this species needs to be assessed at the population level. Eighteen novel polymorphic microsatellite loci for R. uyekii were developed using an enriched partial genomic library. Polymorphisms at these loci were studied in 150 individuals collected from three populations. The number of alleles at each locus ranged from 3 to 47 (mean = 17.1). Within the populations, the observed heterozygosity ranged from 0.032 to 1.000, expected heterozygosity from 0.082 to 0.967, and polymorphism information content from 0.078 to 0.950. Six loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni’s correction, and no significant linkage disequilibrium was detected between most locus pairs, except in three cases. These highly informative microsatellite markers should be useful for genetic population structure analyses of R. uyekii.

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ID Code: 87256
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Rhodeus uyekii, Korean rose bitterling, Microsatellite loci, Genetic diversity
DOI: 10.4238/2014.October.7.9
ISSN: 1676-5680
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2014 FUNPEC-RP
Deposited On: 10 Sep 2015 00:06
Last Modified: 16 Sep 2015 04:26

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