Development of polymorphic microsatellite markers suitable for genetic linkage mapping of olive flounder Paralichthys olivaceus
Kim, Woo-Jin, Shin, Eun-Ha, Kong, Hee Jeong, Nam, Bo-Hye, Kim, Young-Ok, Jung, Hyungtaek, & An, Cheul Min (2013) Development of polymorphic microsatellite markers suitable for genetic linkage mapping of olive flounder Paralichthys olivaceus. Fisheries and Aquatic Sciences, 16(4), pp. 303-309.
Microsatellite markers are important for gene mapping and for marker-assisted selection. Sixty-five polymorphic microsatellite markers were developed with an enriched partial genomic library from olive flounder Paralichthys olivaceus an important commercial fish species in Korea. The variability of these markers was tested in 30 individuals collected from the East Sea (Korea). The number of alleles for each locus ranged from 2 to 33 (mean, 17.1). Observed and expected heterozygosity as well as polymorphism information content varied from 0.313 to 1.000 (mean, 0.788), from 0.323 to 0.977 (mean, 0.820), and from 0.277 to 0.960 (mean, 0.787), respectively. Nine loci showed significant deviation from the Hardy-Weinberg equilibrium after sequential Bonferroni correction. Analysis with MICROCHECKER suggested the presence of null alleles at five of these loci with estimated null allele frequencies of 0.126-0.285. These new microsatellite markers from genomic libraries will be useful for constructing a P. olivaceus linkage map.
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|Item Type:||Journal Article|
|Keywords:||Paralichthys olivaceus, Olive flounder, Microsatellite markers, Linkage map|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2013 The Korean Society of Fisheries and Aquatic Science|
|Copyright Statement:||This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License
permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
|Deposited On:||10 Sep 2015 01:04|
|Last Modified:||16 Sep 2015 04:45|
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