Conserved gene structure and function of interleukin-10 in teleost fish
Nam, Bo-Hye, Moon, Ji-Young, Park, Enu-Hee, Kim, Young-Ok, Kim, Dong-Gyun, Kong, Hee Jeong, Kim, Woo-Jin, Jung, Hyungtaek, Jee, Young Ju, Lee, Sang-Jun, & An, Cheul Min (2014) Conserved gene structure and function of interleukin-10 in teleost fish. Journal of Animal and Veterinary Advances, 13(12), pp. 774-782.
Interleukin-10 (IL-10) is an important immunoregulatory cytokine produced by various types of cells. Researchers describe here the isolation and characterization of olive flounder IL-10 (ofIL-10) cDNA and genomic organization. The ofIL-10 gene encodes a 187 amino acid protein and is composed of a five exon/four intron structure, similar to other known IL-10 genes. The ofIL-10 promoter sequence analysis shows a high level of homology in putative binding sites for transcription factors which are sufficient for transcriptional regulation ofIL-10. Important structural residues are maintained in the ofIL-10 protein including the four cysteines responsible for the two intra-chain disulfide bridges reported for human IL-10 and two extra cysteine residues that exist only in fish species. The phylogenetic analysis clustered ofIL-10 with other fish IL-10s and apart from mammalian IL-10 molecules. Quantitative real-time Polymerase Chain Reaction (PCR) analysis demonstrated ubiquitous ofIL-10 gene expression in the 13 tissues examined. Additionally, the induction of ofIL-10 gene expression was observed in the kidney tissue from olive flounder infected with bacteria (Edawardsiella tarda) or virus (Viral Hemorrhagic Septicemia Virus; VHSV). These data indicate that IL-10 is an important immune regulator that is conserved strictly genomic organization and function during the evolution of vertebrate immunity.
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|Item Type:||Journal Article|
|Keywords:||Interleukin-10, Genomic Organization, Promoter, m RNA Expression Analysis|
|Divisions:||Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Deposited On:||10 Sep 2015 03:28|
|Last Modified:||16 Sep 2015 04:59|
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