In vitro micro propagation of Nicotiana benthamiana via axillary shoots

Deo, Pradeep C., Dugdale, Benjamin, Harding, Robert M., Kato, Maiko, & Dale, James (2015) In vitro micro propagation of Nicotiana benthamiana via axillary shoots. The South Pacific Journal of Natural and Applied Sciences, 32(2), pp. 55-60.

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Axillary shoots of Nicotiana benthamiana were regenerated from nodal explants in two weeks using MS media supplemented with the cytokinin, kinetin (0.5 mg/L), and the auxin, indole-3-butyric acid (IBA) (0.1 mg/L). Ninety two percent of shoots were 2.1-20 mm tall, a size ideal for root induction. After transfer to hormone-free MS they readily produced roots within seven days, with phenotypically normal, fully developed plants being obtained within four weeks. Leaf chlorosis due to iron deficiency was observed in plants over time, however, this was overcome by doubling the concentration of inorganic iron. This rapid micro-propagation system is particularly useful for the in vitro mass production of N. benthamiana plants for various biotechnological applications.

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ID Code: 87275
Item Type: Journal Article
Refereed: Yes
Keywords: nodal explant, kinetin, indole-3-butyric acid
DOI: 10.1071/SP14009
ISSN: 1838-8388
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Past > Schools > School of Life Sciences
Copyright Owner: Copyright 2014 The University of the South Pacific
Deposited On: 07 Sep 2015 03:17
Last Modified: 12 Sep 2015 11:09

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