Ankylosing spondylitis is associated with the anthrax toxin receptor 2 gene (ANTXR2)

Karaderi, T., Keidel, S. M., Pointon, J. J., Appleton, L. H., Brown, M A, Evans, D. M., & Wordsworth, B. P. (2014) Ankylosing spondylitis is associated with the anthrax toxin receptor 2 gene (ANTXR2). Annals of the Rheumatic Diseases, 73(11), pp. 2054-2058.

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Abstract

Objectives

ANTXR2 variants have been associated with ankylosing spondylitis (AS) in two previous genome-wide association studies (GWAS) (p∼9×10-8). However, a genome-wide significant association (p<5×10-8) was not observed. We conducted a more comprehensive analysis of ANTXR2 in an independent UK sample to confirm and refine this association.

Methods

A replication study was carried out with 2978 cases and 8365 controls. Then, these were combined with non-overlapping samples from the two previous GWAS in a meta-analysis. Human leukocyte antigen (HLA)-B27 stratification was also performed to test for ANTXR2-HLA-B27 interaction.

Results

Out of nine single nucleotide polymorphisms (SNP) in the study, five SNPs were nominally associated (p<0.05) with AS in the replication dataset. In the meta-analysis, eight SNPs showed evidence of association, the strongest being with rs12504282 (OR=0.88, p=6.7×10-9). Seven of these SNPs showed evidence for association in the HLA-B27-positive subgroup, but none was associated with HLA-B27-negative AS. However, no statistically significant interaction was detected between HLA-B27 and ANTXR2 variants.

Conclusions

ANTXR2 variants are clearly associated with AS. The top SNPs from two previous GWAS (rs4333130 and rs4389526) and this study (rs12504282) are in strong linkage disequilibrium (r2≥0.76). All are located near a putative regulatory region. Further studies are required to clarify the role played by these ANTXR2 variants in AS.

Impact and interest:

2 citations in Scopus
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8 citations in Web of Science®

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ID Code: 87741
Item Type: Journal Article
Refereed: Yes
Additional Information: No file attached.
Keywords: HLA B27 antigen, ANTXR2 protein, human, receptor, ankylosing spondylitis, ANTXR2 gene, Article, child, controlled study, disease association, gene, gene function, gene linkage disequilibrium, gene locus, genetic association, genetic variability, human, major clinical study, replication study, single nucleotide polymorphism, United Kingdom, case control study, genetic predisposition, genetics, meta analysis, Case-Control Studies, Genetic Predisposition to Disease, Genome-Wide Association Study, HLA-B27 Antigen, Humans, Linkage Disequilibrium, Polymorphism, Single Nucleotide, Receptors, Peptide, Spondylitis, Ankylosing
DOI: 10.1136/annrheumdis-2014-205643
ISSN: 0003-4967
Divisions: Current > Schools > School of Biomedical Sciences
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 24 Sep 2015 04:43
Last Modified: 18 Feb 2016 05:06

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