Brief report: Intestinal dysbiosis in ankylosing spondylitis

Costello, Mary-Ellen, Ciccia, Francesco, Willner, Dana, Warrington, Nicole, Robinson, Philip C., Gardiner, Brooke, Marshall, Mhairi, Kenna, Tony J., Triolo, Giovanni, & Brown, Matthew A. (2015) Brief report: Intestinal dysbiosis in ankylosing spondylitis. Arthritis and Rheumatology, 67(3), pp. 686-691.

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Abstract

Objective

Ankylosing spondylitis (AS) is a common, highly heritable immune-mediated arthropathy that occurs in genetically susceptible individuals exposed to an unknown but likely ubiquitous environmental trigger. There is a close relationship between the gut and spondyloarthritis, as exemplified in patients with reactive arthritis, in whom a typically self-limiting arthropathy follows either a gastrointestinal or urogenital infection. Microbial involvement in AS has been suggested; however, no definitive link has been established. The aim of this study was to determine whether the gut in patients with AS carries a distinct microbial signature compared with that in the gut of healthy control subjects.

Methods

Microbial profiles for terminal ileum biopsy specimens obtained from patients with recent-onset tumor necrosis factor antagonist-naive AS and from healthy control subjects were generated using culture-independent 16S ribosomal RNA gene sequencing and analysis techniques.

Results

Our results showed that the terminal ileum microbial communities in patients with AS differ significantly (P < 0.001) from those in healthy control subjects, driven by a higher abundance of 5 families of bacteria (Lachnospiraceae [P = 0.001], Ruminococcaceae [P = 0.012], Rikenellaceae [P = 0.004], Porphyromonadaceae [P = 0.001], and Bacteroidaceae [P = 0.001]) and a decrease in the abundance of 2 families of bacteria (Veillonellaceae [P = 0.01] and Prevotellaceae [P = 0.004]).

Conclusion

We show evidence for a discrete microbial signature in the terminal ileum of patients with AS compared with healthy control subjects. The microbial composition was demonstrated to correlate with disease status, and greater differences were observed between disease groups than within disease groups. These results are consistent with the hypothesis that genes associated with AS act, at least in part, through effects on the gut microbiome.

Impact and interest:

20 citations in Scopus
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29 citations in Web of Science®

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ID Code: 87787
Item Type: Journal Article
Refereed: Yes
Keywords: interleukin 23, nonsteroid antiinflammatory agent, RNA 16S, Acidaminococcaceae, Actinomyces, Actinomycetaceae, adult, ankylosing spondylitis, Article, Bacteroidaceae, Clostridia, Clostridium, colonoscopy, controlled study, dysbiosis, Faecalibacterium, female, gene sequence, genetic analysis, human, human tissue, ileum, intestine biopsy, intestine flora, Lachnospiraceae, major clinical study, male, microbial community, middle aged, Porphyromonadaceae, Prevotella, Prevotellaceae, priority journal, Rikenellaceae, Ruminococcaceae, Streptococcus, Veillonella, young adult
DOI: 10.1002/art.38967
ISSN: 2326-5191
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2015 by the American College of Rheumatology
Deposited On: 25 Sep 2015 02:58
Last Modified: 21 Mar 2016 04:13

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