Total transcriptome, proteome, and allergome of Johnson grass pollen, which is important for allergic rhinitis in subtropical regions

Campbell, Bradley C., Gilding, Edward K., Timbrell, Victoria, Guru, Preethi, Loo, Dorothy, Zennaro, Danila, Mari, Adriano, Solley, Graham, Hill, Michelle M., Godwin, Ian D., & Davies, Janet M. (2015) Total transcriptome, proteome, and allergome of Johnson grass pollen, which is important for allergic rhinitis in subtropical regions. Journal of Allergy and Clinical Immunology, 135(1), pp. 133-142.

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Abstract

Background

Genomic data are lacking for many allergen sources. To circumvent this limitation, we implemented a strategy to reveal the repertoire of pollen allergens of a grass with clinical importance in subtropical regions, where an increasing proportion of the world's population resides.

Objective

We sought to identify and immunologically characterize the allergenic components of the Panicoideae Johnson grass pollen (JGP; Sorghum halepense).

Methods

The total pollen transcriptome, proteome, and allergome of JGP were documented. Serum IgE reactivities with pollen and purified allergens were assessed in 64 patients with grass pollen allergy from a subtropical region.

Results

Purified Sor h 1 and Sor h 13 were identified as clinically important allergen components of JGP with serum IgE reactivity in 49 (76%) and 28 (43.8%), respectively, of patients with grass pollen allergy. Within whole JGP, multiple cDNA transcripts and peptide spectra belonging to grass pollen allergen families 1, 2, 4, 7, 11, 12, 13, and 25 were identified. Pollen allergens restricted to subtropical grasses (groups 22-24) were also present within the JGP transcriptome and proteome. Mass spectrometry confirmed the IgE-reactive components of JGP included isoforms of Sor h 1, Sor h 2, Sor h 13, and Sor h 23.

Conclusion

Our integrated molecular approach revealed qualitative differences between the allergenic components of JGP and temperate grass pollens. Knowledge of these newly identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with grass pollen allergy in subtropical regions and reduce the burden of allergic respiratory disease globally.

Impact and interest:

15 citations in Scopus
12 citations in Web of Science®
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ID Code: 87897
Item Type: Journal Article
Refereed: Yes
Keywords: allergen components, allergic rhinitis, Allergome, IgE, Johnson grass pollen, pollen allergy, proteome, transcriptome, complementary DNA, immunoglobulin E, isoprotein, pollen antigen, proteome, transcriptome, allergen, plant antigen, plant extract, vegetable protein, adult, allergenicity, Article, controlled study, female, gel electrophoresis, grass pollen, high performance liquid chromatography, human, immunoblotting, immunoglobulin blood level, major clinical study, male, mass spectrometry, multicenter study, nucleotide sequence, perennial ryegrass, prick test, proteomics, respiratory tract disease, serodiagnosis, Sorghum halepense, blood, immunology, middle aged, pollen, skin test, sorghum, tropic climate, Allergens, Antigens, Plant, Humans, Plant Extracts, Plant Proteins, Rhinitis, Allergic, Skin Tests, Tropical Climate
DOI: 10.1016/j.jaci.2014.06.034
ISSN: 0091-6749
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 30 Sep 2015 04:41
Last Modified: 25 Jun 2017 18:01

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