An immunodiagnostic assay for quantitation of specific ige to the major pollen allergen component, pas n 1, of the subtropical bahia grass

Timbrell, V.L., Riebelt, L., Simmonds, C., Solley, G., Smith, W.B., McLean-Tooke, A., Van Nunen, S., Smith, P.K., Upham, J.W., Langguth, D., & Davies, J.M. (2014) An immunodiagnostic assay for quantitation of specific ige to the major pollen allergen component, pas n 1, of the subtropical bahia grass. International Archives of Allergy and Immunology, 165(4), pp. 219-228.

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Abstract

Background

Pollens of the Panicoideae subfamily of grasses including Bahia (Paspalum notatum) are important allergen sources in subtropical regions of the world. An assay for specific IgE to the major molecular allergenic component, Pas n 1, of Bahia grass pollen (BaGP) would have immunodiagnostic utility for patients with pollen allergy in these regions.

Methods

Biotinylated Pas n 1 purified from BaGP was coated onto streptavidin ImmunoCAPs. Subjects were assessed by clinical history of allergic rhinitis and skin prick test (SPT) to aeroallergens. Serum total, BaGP-specific and Pas n 1-specific IgE were measured. Results: Pas n 1 IgE concentrations were highly correlated with BaGP SPT (r = 0.795, p < 0.0001) and BaGP IgE (r = 0.915, p < 0.0001). At 0.23 kU/l Pas n 1 IgE, the diagnostic sensitivity (92.4%) and specificity (93.1%) for the detection of BaGP allergy was high (area under receiver operator curve 0.960, p < 0.0001). The median concentrations of Pas n 1 IgE in non-Atopic subjects (0.01 kU/l, n = 67) and those with other allergies (0.02 kU/l, n = 59) showed no inter-group difference, whilst grass pollen-Allergic patients with allergic rhinitis showed elevated Pas n 1 IgE (6.71 kU/l, n = 182, p < 0.0001). The inter-Assay coefficient of variation for the BaGP-Allergic serum pool was 6.92%.

Conclusions

Pas n 1 IgE appears to account for most of the BaGP-specific IgE. This molecular component immunoassay for Pas n 1 IgE has potential utility to improve the sensitivity and accuracy of diagnosis of BaGP allergy for patients in subtropical regions.

Impact and interest:

2 citations in Scopus
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1 citations in Web of Science®

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ID Code: 87899
Item Type: Journal Article
Refereed: Yes
Keywords: Allergen, Bahia grass pollen, ImmunoCAP, Immunodiagnosis, Pas n 1, Specific IgE, immunoglobulin E, streptavidin, adult, allergic rhinitis, Article, biotinylation, diagnostic accuracy, diagnostic test accuracy study, diagnostic value, female, grass pollen, human, immunoassay, major clinical study, male, Paspalum notatum, pollen allergy, prick test, priority journal, quantitative assay, sensitivity and specificity, serodiagnosis, area under the curve, Australia, blood, clinical trial, cross-sectional study, immunology, middle aged, multicenter study, nonparametric test, Paspalum, pollen, procedures, receiver operating characteristic, Rhinitis, Allergic, Seasonal, standards, Area Under Curve, Cross-Sectional Studies, Humans, Queensland, ROC Curve, Statistics, Nonparametric
DOI: 10.1159/000369341
ISSN: 1018-2438
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 30 Sep 2015 04:18
Last Modified: 01 Oct 2015 00:49

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