Unique and cross-reactive T cell epitope peptides of the major bahia grass pollen allergen, pas n 1
Etto, T., De Boer, C., Prickett, S., Gardner, L.M., Voskamp, A., Davies, J.M., O'Hehir, R.E., & Rolland, J.M. (2012) Unique and cross-reactive T cell epitope peptides of the major bahia grass pollen allergen, pas n 1. International Archives of Allergy and Immunology, 159(4), pp. 355-366.
Bahia grass pollen (BaGP) is a major cause of allergic rhinitis. Subcutaneous allergen-specific immunotherapy is effective for grass pollen allergy, but is unsuitable for patients with moderate to severe asthma due to the risk of anaphylaxis. T cell-reactive but IgE nonreactive peptides provide a safer treatment option. This study aimed to identify and characterize dominant CD4+ T cell epitope peptides of the major BaGP allergen, Pas n 1.
Pas n 1-specific T cell lines generated from the peripheral blood of BaGP-allergic subjects were tested for proliferative and cytokine response to overlapping 20-mer Pas n 1 peptides. Cross-reactivity to homologous peptides from Lol p 1 and Cyn d 1 of Ryegrass and Bermuda grass pollen, respectively, was assessed using Pas n 1 peptide-specific T cell clones. MHC class II restriction of Pas n 1 peptide T cell recognition was determined by HLA blocking assays and peptide IgE reactivity tested by dot blotting.
Three Pas n 1 peptides showed dominant T cell reactivity; 15 of 18 (83%) patients responded to one or more of these peptides. T cell clones specific for dominant Pas n 1 peptides showed evidence of species-specific T cell reactivity as well as cross-reactivity with other group 1 grass pollen allergens. The dominant Pas n 1 T cell epitope peptides showed HLA binding diversity and were non-IgE reactive.
The immunodominant T cell-reactive Pas n 1 peptides are candidates for safe immunotherapy for individuals, including those with asthma, who are allergic to Bahia and possibly other grass pollens.
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|Item Type:||Journal Article|
|Keywords:||Allergic rhinitis, Bahia grass pollen allergy, T cell cross-reactivity, T cell epitopes, allergen, gamma interferon, HLA antigen class 2, HLA DR antigen, interleukin 10, interleukin 4, interleukin 5, peptide, tumor necrosis factor, adult, allele, article, blood, CD4+ T lymphocyte, cell population, clinical article, controlled study, cross reaction, cytokine response, female, grass pollen, HLA system, human, human tissue, immunotherapy, lymphocyte proliferation, male, molecular recognition, priority journal, protein binding, T lymphocyte activation, Allergens, Amino Acid Sequence, Antigens, Plant, Asthma, CD4-Positive T-Lymphocytes, Clone Cells, Cross Reactions, Cynodon, Desensitization, Immunologic, Epitopes, T-Lymphocyte, Humans, Immunoglobulin E, Lolium, Middle Aged, Molecular Sequence Data, Oligopeptides, Paspalum, Plant Proteins, Pollen, Rhinitis, Allergic, Seasonal|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
|Deposited On:||30 Sep 2015 05:17|
|Last Modified:||01 Oct 2015 04:17|
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