Functional immunoglobulin E cross-reactivity between Pas n 1 of Bahia grass pollen and other group 1 grass pollen allergens

Davies, J.M., Dang, T.D., Voskamp, A., Drew, A.C., Biondo, M., Phung, M., Upham, J.W., Rolland, J.M., & O'Hehir, R.E. (2011) Functional immunoglobulin E cross-reactivity between Pas n 1 of Bahia grass pollen and other group 1 grass pollen allergens. Clinical and Experimental Allergy, 41(2), pp. 281-291.

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Abstract

Background

Grass pollens are major triggers of allergic rhinitis and asthma, but the immunological relationships between pollen allergens of the subtropical Bahia grass, Paspalum notatum, and temperate grasses are unresolved.

Objective

To assess serum IgE cross-reactivity between subtropical P. notatum and temperate Lolium perenne (Ryegrass) pollen allergens.

Methods

Serum IgE reactivities of grass pollen-allergic patients with P. notatum, L. perenne and Cynodon dactylon (Bermuda grass) pollen extracts and their respective purified group 1 allergens, Pas n 1, Lol p 1 and Cyn d 1, were compared by immunoblotting, ELISA and basophil activation.

Results

In a cohort of 51 patients from a temperate region, a high frequency of IgE reactivity with each grass pollen was detected, but reactivity with L. perenne pollen was substantially greater than with P. notatum and C. dactylon pollen. Similarly, serum IgE reactivity with Lol p 1 was greater than with Pas n 1 or Cyn d 1. For seven of eight sera studied in detail, asymmetric serum IgE cross-reactivity was observed; L. perenne pollen inhibited IgE reactivity with P. notatum pollen but not the converse, and IgE reactivity with Pas n 1 was inhibited by Lol p 1 but IgE reactivity with Lol p 1 was not inhibited by Pas n 1 or Cyn d 1. Importantly, P. notatum pollen and Pas n 1 activated basophils in grass pollen-allergic patients from a temperate region, although stimulation was greater by pollen of L. perenne than P. notatum or C. dactylon, and by Lol p 1 than Pas n 1 or Cyn d 1. In contrast, a cohort of 47 patients from a subtropical region showed similar IgE reactivity with P. notatum and L. perenne pollen, and reciprocal cross-inhibition of IgE reactivity between L. perenne and P. notatum.

Conclusions

Pollen allergens of the subtropical P. notatum, including Pas n 1, show clinically relevant IgE cross-reactivity with pollen allergens of L. perenne but also species-specific IgE reactivity.

Impact and interest:

19 citations in Scopus
18 citations in Web of Science®
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ID Code: 87910
Item Type: Journal Article
Refereed: Yes
Keywords: Allergic rhinitis, Bahia grass pollen, Grass pollen allergy, IgE cross-reactivity, Pas n 1, Ryegrass pollen, grass pollen allergen, grass pollen extract, immunoglobulin E, pollen antigen, unclassified drug, article, controlled study, cross reaction, Cynodon, enzyme linked immunosorbent assay, grass pollen, human, human cell, immunoblotting, immunoglobulin blood level, major clinical study, Paspalum, perennial ryegrass, pollen allergy, priority journal, Allergens, Cross Reactions, Humans, Lolium, Penicillium, Pollen, Rhinitis, Allergic, Seasonal
DOI: 10.1111/j.1365-2222.2010.03670.x
ISSN: 0954-7894
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 30 Sep 2015 04:55
Last Modified: 25 Jun 2017 18:01

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