Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahia grass Paspalum notatum pollen

Davies, Janet M., Mittag, D., Dang, T. D., Symons, K., Voskamp, A., Rolland, J. M., & O'Hehir, R. E. (2008) Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahia grass Paspalum notatum pollen. Molecular Immunology, 46(2), pp. 286-293.

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Bahia grass, Paspalum notatum, is a clinically important subtropical grass with a prolonged pollination season from spring to autumn. We aimed to clone and characterise the major Bahia grass pollen allergen, Pas n 1. Grass pollen-allergic patients presenting to a tertiary hospital allergy clinic were tested for IgE reactivity with Bahia grass pollen extract by skin prick testing, ImmunoCAP, ELISA and immunoblotting. Using primers deduced from the N-terminal peptide sequence of a group 1 allergen of Bahia grass pollen extract separated by two-dimensional gel electrophoresis, the complete Pas n 1 cDNA was obtained by rapid amplification of cDNA ends and cloned. Biological relevance of recombinant Pas n 1 expressed in Escherichia coli was assessed by serum IgE reactivity and basophil activation. Twenty-nine of 34 (85%) consecutive patients presenting with grass pollen allergy were skin prick test positive to Bahia grass pollen. The Pas n 1 cDNA has sequence homology with the β-expansin 1 glycoprotein family and is more closely related to the maize pollen group 1 allergen (85% identity) than to ryegrass Lol p 1 or Timothy grass Phl p 1 (64 and 66% identity, respectively). rPas n 1 reacted with serum IgE in 47 of 55 (85%) Bahia grass pollen-allergic patients, activated basophils and inhibited serum IgE reactivity with the 29 kDa band of Bahia grass pollen extract. In conclusion the cDNA for the major group 1 allergen of the subtropical Bahia grass pollen, Pas n 1, was identified and cloned. rPas n 1 is immunologically active and is a valuable reagent for diagnosis and specific immunotherapy of grass pollen allergy.

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ID Code: 87915
Item Type: Journal Article
Refereed: Yes
Additional Information: No file attached.
Keywords: Allergic rhinitis, Bahia grass pollen, Group 1 allergen, Pas n 1, Paspalum notatum, glycoprotein, immunoglobulin E, pollen antigen, protein beta expansin 1, unclassified drug, amino acid sequence, antigen expression, article, clinical article, controlled study, enzyme linked immunosorbent assay, Escherichia coli, female, hay fever, human, immunoblotting, immunoglobulin blood level, male, molecular cloning, nonhuman, Poaceae, pollen allergy, priority journal, sequence homology, skin tingling, two dimensional gel electrophoresis, Allergens, Asthma, Base Sequence, Basophils, Cloning, Molecular, DNA, Complementary, Humans, Molecular Sequence Data, Paspalum, Plant Proteins, Pollen, Recombinant Proteins, Rhinitis, Allergic, Seasonal, Skin Tests, Lolium, Phleum pratense, Zea mays
DOI: 10.1016/j.molimm.2008.08.267
ISSN: 0161-5890
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Deposited On: 01 Oct 2015 01:05
Last Modified: 02 Oct 2015 02:11

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