A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction

Suphioglu, C., Schäppi, G., Kenrick, J., Levy, D., Davies, J. M., & O'Hehir, R. E. (2001) A novel grass pollen allergen mimotope identified by phage display peptide library inhibits allergen-human IgE antibody interaction. FEBS Letters, 502(1-2), pp. 46-52.

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Abstract

The aim of this study was to investigate the molecular basis of human IgE-allergen interaction by screening a phage-displayed peptide library with an allergen-specific human IgE-mimicking monoclonal antibody (mAb). A mAb that reacted with major grass pollen allergens was successfully identified and shown to inhibit human IgE-allergen interaction. Biopanning of a phage-displayed random peptide library with this mAb yielded a 12 amino acid long mimotope. A synthetic peptide based on this 12-mer mimotope inhibited mAb and human IgE binding to grass pollen extracts. Our results indicate that such synthetic peptide mimotopes of allergens have potential as novel therapeutic agents. © 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Impact and interest:

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16 citations in Web of Science®

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ID Code: 87920
Item Type: Journal Article
Refereed: Yes
Keywords: Allergen, Epitope, Grass pollen, Human immunoglobulin E, Mimotope, Phage display, amino acid, antiallergic agent, immunoglobulin E, immunoglobulin E antibody, monoclonal antibody, peptide library, pollen antigen, pollen extract, synthetic peptide, allergy, antigen binding, antigen specificity, article, controlled study, cross linking, DNA sequence, drug effect, drug mechanism, drug synthesis, grass, human, hypersensitivity reaction, molecular interaction, priority journal, sequence analysis, Allergens, Animals, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Epitopes, B-Lymphocyte, Humans, Immunoblotting, Mice, Mice, Inbred BALB C, Peptides, Poaceae, Pollen
DOI: 10.1016/S0014-5793(01)02661-8
ISSN: 0014-5793
Copyright Owner: Copyright 2001 Elsevier BV
Deposited On: 30 Sep 2015 05:25
Last Modified: 30 Sep 2015 05:25

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