Rheumatoid arthritis sera react with a phage-displayed peptide selected by a monoclonal antibody to type II collagen that has homology to EBNA-1

Davies, J. M., Mackay, I. R., & Rowley, M. J. (1999) Rheumatoid arthritis sera react with a phage-displayed peptide selected by a monoclonal antibody to type II collagen that has homology to EBNA-1. Autoimmunity, 30(1), pp. 53-59.

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Antibodies to type II collagen, and to Epstein Barr virus nuclear antigen-1 (EBNA-1) have been associated with rheumatoid arthritis (RA). In studies involving probing of phage-displayed random peptide libraries with an antibody to type II collagen, CII-C1, we observed that among 17 phagotopes selected 5 expressed peptides with homology with the sequence of EBNA-1. The residues in common were RLPFG. Hence we tested sera from 50 patients with RA, of whom 26 had antibodies to native type II collagen, and 43 healthy controls, for reactivity by ELISA with a phagotope selected 4 times, which expressed the peptide RRLPFGSQM. Eight RA sera (16%) but no normal sera reacted with the phagotope (p = 0.025). This reactivity could not be correlated with reactivity of RA sera with EBNA-1 by semi-quantitative western blot, with which reactivity occurred in 78% of RA patients and 81% of controls. Evidence for molecular mimicry was not found insofar as the phagotope did not inhibit reactivity of RA sera with EBNA-1 and CII-C1 was not reactive with EBNA-1. We conclude that the reactivity of the RA sera with the phagotope is most likely due to the phagotope being a mimic of an epitope of type II collagen for a proportion of RA sera.

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ID Code: 87931
Item Type: Journal Article
Refereed: Yes
Keywords: Epstein Barr virus, Molecular mimicry, Phage display, Rheumatoid arthritis, Type II collagen, collagen antibody, collagen type 2, epstein barr virus antigen, monoclonal antibody, adult, aged, article, clinical article, controlled study, enzyme linked immunosorbent assay, female, human, male, western blotting, Aged, 80 and over, Antibodies, Monoclonal, Arthritis, Rheumatoid, Blotting, Western, Cells, Cultured, Chemiluminescent Measurements, Collagen, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epstein-Barr Virus Nuclear Antigens, Humans, Middle Aged, Peptide Library, Sequence Homology, Amino Acid
DOI: 10.3109/08916939908994760
ISSN: 08916934
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: Copyright 1999 OPA (Overseas Publishers Association) N.V
Deposited On: 01 Oct 2015 02:34
Last Modified: 01 Oct 2015 02:34

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