Mimotopes identified by phage display for the monoclonal antibody CII- C1 to type II collagen

Cook, Andrew D., Davies, Janet M., Myers, Mark A., Mackay, Ian R., & Rowley, Merrill J. (1998) Mimotopes identified by phage display for the monoclonal antibody CII- C1 to type II collagen. Journal of Autoimmunity, 11(3), pp. 205-211.

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Abstract

The characterization of B cell epitopes has been advanced by the use of random peptide libraries displayed within the coat protein of bacteriophage. This technique was applied to the monoclonal antibody (mAb) C1 to type II collagen (CII-C1). CII-C1 is known to react with a conformational epitope on type II collagen that includes residues 359-363. Three rounds of selection were used to screen two random nonameric phage libraries and 18 phagotopes were isolated. CII-C1 reacted by ELISA with 17 of the 18 phagotopes: one phagotope contained a stop codon. Of the eight most reactive phage, seven inhibited the reactivity by ELISA of CII-C1 with type II collagen. Of the 18 phage isolated, 11 encoded the motif F-G-x-Q with the sequence F-G-S-Q in 6, 2 encoded F-G-Q, and one the reverse motif Q-x-y-F. Most phagotopes that inhibited the reactivity of CII-C1 encoded two particular motifs consisting of two basic amino acid residues and a hydrophobic residue in the first part of the insert and the F-G-x-Q or F-G-Q motif ill the second part; phagotopes which contained only one basic residue in the first part of the sequence were less reactive. These motifs are not represented in the linear sequence of type II collagen and thus represent mimotopes of the epitope for CII-C1 on type II collagen. There were five phagotopes with peptide inserts containing the sequence RLPFG occurring in the Epstein-Barr virus nuclear antigen, EBNA- 1. This is of interest because EBV has been implicated in the initiation of rheumatoid arthritis (RA) by reason of increased reactivity to EBNA-1 in RA sera. In conclusion, the phage display technique disclosed mimotopes for a conformational epitope of type II collagen, and revealed an interesting homology with a sequence of the EBNA-1 antigen from Epstein Barr virus.

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ID Code: 87932
Item Type: Journal Article
Refereed: Yes
Keywords: Collagen antibodies, Epitopes, Molecular mimicry, Phage display, Rheumatoid arthritis, collagen type 2, epitope, monoclonal antibody, animal cell, antigen recognition, article, binding site, collagen synthesis, conformational transition, dna sequence, enzyme linked immunosorbent assay, molecular interaction, nonhuman, peptide synthesis, priority journal, protein processing, triple helix, Animals, Antibodies, Monoclonal, Antibody Specificity, Arthritis, Autoantibodies, Autoantigens, Bacteriophages, Chickens, Collagen, Enzyme-Linked Immunosorbent Assay, Mice, Mice, Inbred DBA, Oligopeptides, Peptide Library, Sequence Analysis, DNA
DOI: 10.1006/jaut.1998.0200
ISSN: 0896-8411
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1998 Elsevier B.V.
Deposited On: 02 Oct 2015 03:04
Last Modified: 25 Jun 2017 18:01

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