Antibody reactivity to the transmembrane protein of the caprine arthritis encephalitis virus correlates with severity of arthritis: No evidence for the involvement of epitope mimicry
Davies, J.M.., Robinson, W. F., & Carnegie, P. R. (1997) Antibody reactivity to the transmembrane protein of the caprine arthritis encephalitis virus correlates with severity of arthritis: No evidence for the involvement of epitope mimicry. Veterinary Immunology and Immunopathology, 60(1-2), pp. 131-147.
Serum and synovial antibody reactivities of caprine arthritis encephalitis virus (CAEV) infected goats were assessed by Western blotting against purified CAEV antigen and the greatest intensity of reactivity in the serum of arthritic goats was to the gp45 transmembrane protein (TM). The extracytoplasmic domain of the TM gene was cloned into a pGEX vector and expressed in Escherichia coil as a glutathione S transferase fusion protein (GST-TM). This clone was found to be 90.5 and 89.2% homologous to published sequences of CAEV TM gene. Serum of 16 goats naturally infected with CAEV were examined by Western blotting for reactivity to the fusion protein. Antibody reactivity to the GST-TM correlated with clinically detectable arthritis (R = 0.642, P ≤ 0.007). The hypothesis that the immune response to the envelope proteins of the CAEV contributes to the severity of arthritis in goats naturally infected with CAEV via epitope mimicry was tested. Antibodies from 5 CAEV infected goats were affinity purified against the GST-TM fusion protein and tested for cross-reactivity with a series of goat synovial extracts and proteogylcans. No serum antibody response or cross-reactivity of affinity purified antibodies could be detected. Peptides of the CAEV SU that were predicted to be linear epitopes and a similar heat shock protein 83 (HSP) peptide identified by database searching, were synthesized and tested for reactivity in CAEV goats using ELISA, in vitro lymphocyte proliferation and delayed type hypersensitivity (DTH) assays. Peripheral blood lymphocytes from 10 of 17 goats with long term natural CAEV infections proliferated in vitro in response to CAEV and in vivo 3 of 7 CAEV infected goats had a DTH reaction to CAEV antigen. However, none of the peptides elicited significant cell mediated immune responses from CAEV infected goats. No antibody reactivity to the SU peptides or HSP peptide was found. We observed that the antibody reactivity to the CAEV TM protein associated with severity of arthritis however epitope mimicry by the envelope proteins of CAEV is unlikely to be involved.
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|Item Type:||Journal Article|
|Additional Information:||No file attached.|
|Keywords:||Arthritis, Caprine arthritis encephalitis virus, Cross- reactivity, Epitope mimicry, Lentivirus, epitope, glutathione transferase, heat shock protein, hybrid protein, proteoglycan, virus antibody, virus antigen, virus envelope protein, animal cell, animal experiment, animal model, animal tissue, antibody response, article, cellular immunity, controlled study, cross reaction, delayed hypersensitivity, disease severity, goat, lymphocyte proliferation, nonhuman, peripheral lymphocyte, Animals, Antibodies, Viral, Antigens, Viral, Arthritis, Infectious, Arthritis-Encephalitis Virus, Caprine, Cloning, Molecular, Cross Reactions, Epitopes, Goats, Heat-Shock Proteins, Lentivirus Infections, Protozoan Proteins, Viral Proteins, Animalia, Capra, Capra hircus, Caprine arthritis-encephalitis virus, Escherichia|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
|Deposited On:||30 Sep 2015 23:14|
|Last Modified:||01 Oct 2015 00:04|
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