Decellularized periodontal ligament cell sheets with recellularization potential

Farag, A, Vaquette, C, Theodoropoulos, C, Hamlet, S.M., Hutmacher, D.W., & Ivanovski, S (2014) Decellularized periodontal ligament cell sheets with recellularization potential. Journal of Dental Research, 93(12), pp. 1313-1319.

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Abstract

The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets onto melt electrospun polycaprolactone membranes and subsequent bi-directional perfusion with NH4OH/Triton X-100 and DNase solutions. The protocol was shown to remove 92% of DNA content. The structural integrity of the decellularized cell sheets was confirmed by a collagen quantification assay, immunostaining of human collagen type I and fibronectin, and scanning electron microscopy. ELISA was used to demonstrate the presence of residual basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF) in the decellularized cell sheet constructs. The decellularized cell sheets were shown to have the ability to support recellularization by allogenic human periodontal ligament cells. This study describes the fabrication of decellularized periodontal ligament cell sheets that retain an intact extracellular matrix and resident growth factors and can support repopulation by allogenic cells. The decellularized hPDL cell sheet concept has the potential to be utilized in future "off-the-shelf" periodontal tissue engineering strategies.

Impact and interest:

5 citations in Scopus
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8 citations in Web of Science®

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ID Code: 88504
Item Type: Journal Article
Refereed: Yes
Keywords: extracellular matrix, fibroblast growth factor, vascular endothelial growth factor, collagen, tissue engineering, polycaprolactone
DOI: 10.1177/0022034514547762
ISSN: 1544-0591
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 20 Oct 2015 05:28
Last Modified: 20 Oct 2015 05:28

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