Mobile gene silencing in Arabidopsis is regulated by hydrogen peroxide

Liang, Dacheng, White, Rosemary G., & Waterhouse, Peter M. (2014) Mobile gene silencing in Arabidopsis is regulated by hydrogen peroxide. PeerJ, 2, e701.

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Abstract

In plants and nematodes, RNAi can spread from cells from which it is initiated to other cells in the organism. The underlying mechanism controlling the mobility of RNAi signals is not known, especially in the case of plants. A genetic screen designed to recover plants impaired in the movement but not the production or effectiveness of the RNAi signal identified RCI3, which encodes a hydrogen peroxide (H2O2)-producing type III peroxidase, as a key regulator of silencing mobility in Arabidopsis thaliana. Silencing initiated in the roots of rci3 plants failed to spread into leaf tissue or floral tissue. Application of exogenous H2O2 reinstated the spread in rci3 plants and accelerated it in wild-type plants. The addition of catalase or MnO2, which breaks down H2O2, slowed the spread of silencing in wild-type plants. We propose that endogenous H2O2, under the control of peroxidases, regulates the spread of gene silencing by altering plasmodesmata permeability through remodelling of local cell wall structure, and may play a role in regulating systemic viral defence.

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ID Code: 88538
Item Type: Journal Article
Refereed: Yes
Keywords: Arabidopsis, Catalase, Cell-to-cell transport, Mobile silencing, Peroxide, Plasmodesmata, RNAi, Small RNA, Type III peroxidase
DOI: 10.7717/peerj.701
ISSN: 2167-8359
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: 2014 Liang et al.
Deposited On: 02 Nov 2015 01:21
Last Modified: 07 Dec 2016 01:33

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