Droplet digital PCR as a useful tool for the quantitative detection of Enterovirus 71
Lui, Edmund Yan Long & Tan, Eng Lee (2014) Droplet digital PCR as a useful tool for the quantitative detection of Enterovirus 71. Journal of Virological Methods, 207, pp. 200-203.
Hand, foot and mouth disease (HFMD) is a contagious viral disease that frequently affects infants and children and present with blisters and flu-like symptoms. This disease is caused by a group of enteroviruses such as enterovirus 71 (EV71) and coxsackievirus A16 (CA16). However, unlike other HFMD causing enteroviruses, EV71 have also been shown to be associated with more severe clinical manifestation such as aseptic meningitis, brainstem and cerebellar encephalitis which may lead to cardiopulmonary failure and death. Clinically, HFMD caused by EV71 is indistinguishable from other HFMD causing enteroviruses such as CA16. Molecular diagnosis methods such as the use of real-time PCR has been used commonly for the identification of EV71. In this study, two platforms namely the real-time PCR and the droplet digital PCR were compared for the detection quantitation of known EV71 viral copy number. The results reveal accurate and consistent results between the two platforms. In summary, the droplet digital PCR was demonstrated to be a promising technology for the identification and quantitation of EV71 viral copy number.
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|Item Type:||Journal Article|
|Keywords:||Droplet digital PCR; Quantitative real-time PCR; Enterovirus 71; Hand, foot and mouth disease|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
|Copyright Owner:||Copyright 2014 Elsevier B.V.|
|Deposited On:||09 Nov 2015 23:39|
|Last Modified:||09 Nov 2015 23:39|
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