Dependence of light attenuation and backscattering on collagen concentration and chondrocyte density in agarose scaffolds

Puhakka, P H, Ylarinne, J H, Lammi, M J, Saarakkala, S, Tiitu, V, Kroger, H, Viren, T, Jurvelin, J S, & Toyras, J (2014) Dependence of light attenuation and backscattering on collagen concentration and chondrocyte density in agarose scaffolds. Physics in Medicine and Biology, 59(21), pp. 6537-6548.

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Abstract

Optical coherence tomography (OCT) has been applied for high resolution imaging of articular cartilage. However, the contribution of individual structural elements of cartilage on OCT signal has not been thoroughly studied. We hypothesize that both collagen and chondrocytes, essential structural components of cartilage, act as important light scatterers and that variation in their concentrations can be detected by OCT through changes in backscattering and attenuation. To evaluate this hypothesis, we established a controlled model system using agarose scaffolds embedded with variable collagen concentrations and chondrocyte densities. Using OCT, we measured the backscattering coefficient (µb) and total attenuation coefficient (µt) in these scaffolds. Along our hypothesis, light backscattering and attenuation in agarose were dependent on collagen concentration and chondrocyte density. Significant correlations were found between µt and chondrocyte density (ρ = 0.853, p < 0.001) and between µt and collagen concentration (ρ = 0.694, p < 0.001). µb correlated significantly with chondrocyte density (ρ = 0.504, p < 0.001) but not with collagen concentration (ρ = 0.103, p = 0.422) of the scaffold. Thus, quantitation of light backscattering and, especially, attenuation could be valuable when evaluating the integrity of soft tissues, such as articular cartilage with OCT.

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2 citations in Scopus
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3 citations in Web of Science®

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ID Code: 88561
Item Type: Journal Article
Refereed: Yes
Keywords: articular cartilage, attenuation, backscattering, chondrocyte, collagen, optical coherence tomography
DOI: 10.1088/0031-9155/59/21/6537
ISSN: 0031-9155
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 29 Oct 2015 03:32
Last Modified: 29 Oct 2015 03:32

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