The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers

Hariyadi, D.M., Ma, Y., Wang, Y., Bostrom, T., Malouf, J., Turner, M.S., Bhandari, B., & Coombes, A.G.A. (2014) The potential for production of freeze-dried oral vaccines using alginate hydrogel microspheres as protein carriers. Journal of Drug Delivery Science and Technology, 24(2), pp. 178-184.

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Oral administration of dry vaccine formulations is acknowledged to offer major clinical and logistical benefits by eliminating the cold chain required for liquid preparations. A model antigen, bovine serum albumin (BSA) was encapsulated in alginate microspheres using aerosolisation. Hydrated microspheres 25 to 65 μm in size with protein loading of 3.3 % w/w were obtained. Environmental scanning electron microscopy indicated a stabilizing effect of encapsulated protein on alginate hydrogels revealed by an increase in dehydration resistance. Freeze drying of alginate microspheres without use of a cryoprotectant resulted in fragmentation and subsequent rapid loss of the majority of the protein load in simulated intestinal fluid in 2 h, whereas intact microspheres were observed following freeze-drying of BSA-loaded microspheres in the presence of maltodextrin. BSA release from freeze-dried preparations was limited to less than 7 % in simulated gastric fluid over 2 h, while 90 % of the protein load was gradually released in simulated intestinal fluid over 10 h. SDS-PAGE analysis indicated that released BSA largely preserved its molecular weight. These findings demonstrate the potential for manufacturing freeze-dried oral vaccines using alginate microspheres.

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ID Code: 88565
Item Type: Journal Article
Refereed: Yes
Keywords: Oral vaccine; Alginate hydrogel microspheres; Maltodextrin cryoprotectant; Protein; Bovine serum albumin; ESEM; In vitro release
DOI: 10.1016/S1773-2247(14)50029-9
ISSN: 1773-2247
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 09 Nov 2015 00:31
Last Modified: 09 Nov 2015 00:31

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