Single molecule microscopy in 3D cell cultures and tissues

Lauer, Florian, Kammerer, Elke, & Meckel, Tobias (2014) Single molecule microscopy in 3D cell cultures and tissues. Advanced Drug Delivery Reviews, 79-80, pp. 79-94.

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Abstract

From the onset of the first microscopic visualization of single fluorescent molecules in living cells at the beginning of this century, to the present, almost routine application of single molecule microscopy, the method has well-proven its ability to contribute unmatched detailed insight into the heterogeneous and dynamic molecular world life is composed of. Except for investigations on bacteria and yeast, almost the entire story of success is based on studies on adherent mammalian 2D cell cultures. However, despite this continuous progress, the technique was not able to keep pace with the move of the cell biology community to adapt 3D cell culture models for basic research, regenerative medicine, or drug development and screening. In this review, we will summarize the progress, which only recently allowed for the application of single molecule microscopy to 3D cell systems and give an overview of the technical advances that led to it. While initially posing a challenge, we finally conclude that relevant 3D cell models will become an integral part of the on-going success of single molecule microscopy.

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ID Code: 88610
Item Type: Journal Article
Refereed: Yes
Keywords: Single molecule microscopy, 3D cell cultures, Spheroids, Light sheet illumination, HILO illumination, Two-photon microscopy, Multiphoton microscopy, Adaptive optics
DOI: 10.1016/j.addr.2014.10.008
ISSN: 0169-409X
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 05 Nov 2015 04:56
Last Modified: 05 Nov 2015 04:56

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