An improved protocol for the isolation of total genomic DNA from Labyrinthulomycetes

Ranasinghe, Chaminda, Harding, Rob, & Hargreaves, Megan (2015) An improved protocol for the isolation of total genomic DNA from Labyrinthulomycetes. Biotechnology Letters, 37(3), pp. 685-690.

View at publisher


Many protocols have been used for extraction of DNA from Thraustochytrids. These generally involve the use of CTAB, phenol/chloroform and ethanol. They also feature mechanical grinding, sonication, N2 freezing or bead beating. However, the resulting chemical and physical damage to extracted DNA reduces its quality. The methods are also unsuitable for large numbers of samples. Commercially-available DNA extraction kits give better quality and yields but are expensive. Therefore, an optimized DNA extraction protocol was developed which is suitable for Thraustochytrids to both minimise expensive and time-consuming steps prior to DNA extraction and also to improve the yield. The most effective method is a combination of single bead in TissueLyser (Qiagen) and Proteinase K. Results were conclusive: both the quality and the yield of extracted DNA were higher than with any other method giving an average yield of 8.5 µg/100 mg biomass.

Impact and interest:

1 citations in Scopus
Search Google Scholar™
1 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 88654
Item Type: Journal Article
Refereed: Yes
Keywords: DNA extraction Labyrinthulomycetes Marine protists Ω-3 polyunsaturated fatty acids (PUFAs) Thraustochytrids
DOI: 10.1007/s10529-014-1712-1
ISSN: 0141-5492
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2014 Springer Science+Business Media Dordrecht
Deposited On: 03 Nov 2015 23:17
Last Modified: 30 May 2016 23:31

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page