Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells

Zhang, Xufang, Jiang, Hongwei, Gong, Qimei, Fan, Chen, Huang, Yihua, & Ling, Junqi (2014) Expression of high mobility group box 1 in inflamed dental pulp and its chemotactic effect on dental pulp cells. Biochemical and Biophysical Research Communications, 450(4), pp. 1547-1552.

View at publisher

Abstract

High mobility group box 1 protein (HMGB1) is a chromatin protein which can be released extracellularly, eliciting a pro-inflammatory response and promoting tissue repair process. This study aimed to examine the expression and distribution of HMGB1 and its receptor RAGE in inflamed dental pulp tissues, and to assess its effects on proliferation, migration and cytoskeleton of cultured human dental pulp cells (DPCs). Our data demonstrated that cytoplasmic expression of HMGB1 was observed in inflamed pulp tissues, while HMGB1 expression was confined in the nuclei in healthy dental pulp. The mRNA expression of HMGB1 and RAGE were significantly increased in inflamed pulps. In in vitro cultured DPCs, expression of HMGB1 in both protein and mRNA level was up-regulated after treated with lipopolysaccharide (LPS). Exogenous HMGB1 enhanced DPCs migration in a dose-dependent manner and induced the reorganization of f-actin in DPCs. Our results suggests that HMGB1 are not only involved in the process of dental pulp inflammation, but also play an important role in the recruitment of dental pulp stem cells, promoting pulp repair and regeneration.

Impact and interest:

4 citations in Scopus
Search Google Scholar™
4 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 88718
Item Type: Journal Article
Refereed: Yes
Keywords: High mobility group box 1; Receptor for advanced glycation end products; Dental pulp cells; Cell migration; Inflammation
DOI: 10.1016/j.bbrc.2014.07.027
ISSN: 0006-291X
Deposited On: 03 Nov 2015 23:58
Last Modified: 03 Nov 2015 23:58

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page