Tissue requirements for establishing long-term CD4+ T cell-mediated immunity following Leishmania donovani infection
Bunn, Patrick, Stanley, Amanda C., de Labastida Rivera, Fabian, Mulherin, Alexander, Sheel, Meru, Alexander, Clare E., Faleiro, Rebecca J., Amante, Fiona H., Montes De Oca, Marcela, Best, Shannon E., James, Kylie R., Kaye, Paul M., Haque, Ashraful, & Engwerda, Christian R. (2014) Tissue requirements for establishing long-term CD4+ T cell-mediated immunity following Leishmania donovani infection. Journal of Immunology, 192(8), pp. 3709-3718.
Organ-specific immunity is a feature of many infectious diseases, including visceral leishmaniasis caused by Leishmania donovani. Experimental visceral leishmaniasis in genetically susceptible mice is characterized by an acute, resolving infection in the liver and chronic infection in the spleen. CD4+ T cell responses are critical for the establishment and maintenance of hepatic immunity in this disease model, but their role in chronically infected spleens remains unclear. In this study, we show that dendritic cells are critical for CD4+ T cell activation and expansion in all tissue sites examined. We found that FTY720-mediated blockade of T cell trafficking early in infection prevented Ag-specific CD4+ T cells from appearing in lymph nodes, but not the spleen and liver, suggesting that early CD4+ T cell priming does not occur in liver-draining lymph nodes. Extended treatment with FTY720 over the first month of infection increased parasite burdens, although this associated with blockade of lymphocyte egress from secondary lymphoid tissue, as well as with more generalized splenic lymphopenia. Importantly, we demonstrate that CD4+ T cells are required for the establishment and maintenance of antiparasitic immunity in the liver, as well as for immune surveillance and suppression of parasite outgrowth in chronically infected spleens. Finally, although early CD4+ T cell priming appeared to occur most effectively in the spleen, we unexpectedly revealed that protective CD4+ T cell-mediated hepatic immunity could be generated in the complete absence of all secondary lymphoid tissues.
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|Item Type:||Journal Article|
|Divisions:||Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
|Deposited On:||30 Oct 2015 03:04|
|Last Modified:||30 Oct 2015 03:04|
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