Enhanced chondrogenesis of human nasal septum derived progenitors on nanofibrous scaffolds

Shafiee, Abbas, Seyedjafari, Ehsan, Taherzadeh, Elham Sadat, Dinarvand, Peyman, Soleimani, Masoud, & Ai, Jafar (2014) Enhanced chondrogenesis of human nasal septum derived progenitors on nanofibrous scaffolds. Materials Science and Engineering C: Materials for Biological Applications, 40, pp. 445-454.

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Topographical cues can be exploited to regulate stem cell attachment, proliferation, differentiation and function in vitro and in vivo. In this study, we aimed to investigate the influence of different nanofibrous topographies on the chondrogenic differentiation potential of nasal septum derived progenitors (NSP) in vitro. Aligned and randomly oriented Ploy (L-lactide) (PLLA)/Polycaprolactone (PCL) hybrid scaffolds were fabricated via electrospinning. First, scaffolds were fully characterized, and then NSP were seeded on them to study their capacity to support stem cell attachment, proliferation and chondrogenic differentiation. Compared to randomly oriented nanofibers, aligned scaffolds showed a high degree of nanofiber alignment with much better tensile strength properties. Both scaffolds supported NSP adhesion, proliferation and chondrogenic differentiation. Despite the higher rate of cell proliferation on random scaffolds, a better chondrogenic differentiation was observed on aligned nanofibers as deduced from higher expression of chondrogenic markers such as collagen type II and aggrecan on aligned scaffolds. These findings demonstrate that electrospun constructs maintain NSP proliferation and differentiation, and that the aligned nanofibrous scaffolds can significantly enhance chondrogenic differentiation of nasal septum derived progenitors

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7 citations in Scopus
6 citations in Web of Science®
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ID Code: 88765
Item Type: Journal Article
Refereed: Yes
Keywords: Electrospinning, Cartilage, Topography, Nanofiber, Nasal septum derived progenitor
DOI: 10.1016/j.msec.2014.04.027
ISSN: 0928-4931
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Copyright Owner: © 2014 Elsevier B.V
Deposited On: 29 Oct 2015 04:40
Last Modified: 25 Jun 2017 22:01

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