Histopathological features of bone regeneration in a canine segmental ulnar defect model

Hobbenaghi, Rahim, Mahboob, Pariya, Saifzadeh, Siamak, Javanbakht, Javad, Manesh, Javad Yaghoobi Yeganeh, Mortezaee, Rasool, Touni, Seyed Rashid, Hosseini, Ehsan, Aghajanshaker, Shahin, Moloudizargari, Milad, & Javaherypour, Soheil (2014) Histopathological features of bone regeneration in a canine segmental ulnar defect model. Diagnostic Pathology, 9(59).

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Abstract

Background

Today, finding an ideal biomaterial to treat the large bone defects, delayed unions and non-unions remains a challenge for orthopaedic surgeions and researchers. Several studies have been carried out on the subject of bone regeneration, each having its own advantages. The present study has been designed in vivo to evaluate the effects of cellular auto-transplantation of tail vertebrae on healing of experimental critical bone defect in a dog model.

Methods

Six indigenous breeds of dog with 32 ± 3.6 kg average weight from both sexes (5 males and 1 female) received bilateral critical-sized ulnar segmental defects. After determining the health condition, divided to 2 groups: The Group I were kept as control I (n = 1) while in Group II (experimental group; n = 5) bioactive bone implants were inserted. The defects were implanted with either autogeneic coccygeal bone grafts in dogs with 3-4 cm diaphyseal defects in the ulna. Defects were stabilized with internal plate fixation, and the control defects were not stabilized. Animals were euthanized at 16 weeks and analyzed by histopathology.

Results

Histological evaluation of this new bone at sixteen weeks postoperatively revealed primarily lamellar bone, with the formation of new cortices and normal-appearing marrow elements. And also reformation cortical compartment and reconstitution of marrow space were observed at the graft-host interface together with graft resorption and necrosis responses. Finally, our data were consistent with the osteoconducting function of the tail autograft.

Conclusions

Our results suggested that the tail vertebrae autograft seemed to be a new source of autogenous cortical bone in order to supporting segmental long bone defects in dogs. Furthermore, cellular autotransplantation was found to be a successful replacement for the tail vertebrae allograft bone at 3-4 cm segmental defects in the canine mid- ulna. Clinical application using graft expanders or bone autotransplantation should be used carefully and requires further investigation.

Impact and interest:

6 citations in Scopus
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5 citations in Web of Science®

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ID Code: 88782
Item Type: Journal Article
Refereed: Yes
Keywords: Bone defect, Bone regeneration, Vascular bone grafting, Histopathology, Dog
DOI: 10.1186/1746-1596-9-59
ISSN: 1746-1596
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2014 Hobbenaghi et al.; licensee BioMed Central Ltd.
Copyright Statement: This is an Open Access article distributed under the terms of the
Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public
Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this
article, unless otherwise stated.
Deposited On: 21 Oct 2015 03:12
Last Modified: 21 Oct 2015 03:13

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