SERS-barcoded colloidal gold NP assemblies as imaging agents for use in biodiagnostics

Dey, Priyanka, Olds, William, Blakey, Idriss, Thurecht, Kristofer, Kiriakous, Emad, & Fredericks, Peter M. (2014) SERS-barcoded colloidal gold NP assemblies as imaging agents for use in biodiagnostics. In Mahadevan-Jansen, A. & Petrich, W. (Eds.) Proceddings of SPIE 8939: Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, Society of Photo-Optical Instrumentation Engineers, San Francisco, CA, 89390A-1---10.

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There is a growing need for new biodiagnostics that combine high throughput with enhanced spatial resolution and sensitivity. Gold nanoparticle (NP) assemblies with sub-10 nm particle spacing have the benefits of improving detection sensitivity via Surface enhanced Raman scattering (SERS) and being of potential use in biomedicine due to their colloidal stability. A promising and versatile approach to form solution-stable NP assemblies involves the use of multi-branched molecular linkers which allows tailoring of the assembly size, hot-spot density and interparticle distance. We have shown that linkers with multiple anchoring end-groups can be successfully employed as a linker to assemble gold NPs into dimers, linear NP chains and clustered NP assemblies. These NP assemblies with diameters of 30-120 nm are stable in solution and perform better as SERS substrates compared with single gold NPs, due to an increased hot-spot density. Thus, tailored gold NP assemblies are potential candidates for use as biomedical imaging agents. We observed that the hot-spot density and in-turn the SERS enhancement is a function of the linker polymer concentration and polymer architecture. New deep Raman techniques like Spatially Offset Raman Spectroscopy (SORS) have emerged that allow detection from beneath diffusely scattering opaque materials, including biological media such as animal tissue. We have been able to demonstrate that the gold NP assemblies could be detected from within both proteinaceous and high lipid containing animal tissue by employing a SORS technique with a backscattered geometry.

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ID Code: 88831
Item Type: Conference Paper
Refereed: Yes
DOI: 10.1117/12.2037159
ISBN: 9780819498526
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 20 Oct 2015 01:06
Last Modified: 20 Oct 2015 01:08

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