Analysis of focal adhesion turnover: A quantitative live-cell imaging example

Stehbens, Samantha & Wittmann, Torsten (2014) Analysis of focal adhesion turnover: A quantitative live-cell imaging example. Methods in Cell Biology, 123, pp. 335-346.

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Recent advances in optical and fluorescent protein technology have rapidly raised expectations in cell biology, allowing quantitative insights into dynamic intracellular processes like never before. However, quantitative live-cell imaging comes with many challenges including how best to translate dynamic microscopy data into numerical outputs that can be used to make meaningful comparisons rather than relying on representative data sets. Here, we use analysis of focal adhesion turnover dynamics as a straightforward specific example on how to image, measure, and analyze intracellular protein dynamics, but we believe this outlines a thought process and can provide guidance on how to understand dynamic microcopy data of other intracellular structures.

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3 citations in Scopus
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2 citations in Web of Science®

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ID Code: 88843
Item Type: Journal Article
Refereed: Yes
Keywords: Cell migration; Curve fitting; Fluorescence microscopy; Fluorescent proteins; Focal adhesion dynamics; Image analysis; Quantitative microscopy; Spinning disk confocal microscopy
DOI: 10.1016/B978-0-12-420138-5.00018-5
ISSN: 0091-679X
Deposited On: 22 Oct 2015 03:47
Last Modified: 22 Oct 2015 03:48

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