Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging
Susaki, Etsuo A, Tainaka, Kazuki, Perrin, Dimitri, Yukinaga, Hiroko, Kuno, Akihiro, & Ueda, Hiroki R (2015) Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging. Nature Protocols, 10(11), pp. 1709-1727.
Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples. The organ or body is cleared by immersion for 1â€“14 d, with the exact time required dependent on the sample type and the experimental purposes. A single imaging set can be completed in 30â€“60 min. Image processing and analysis can take <1 d, but it is dependent on the number of samples in the data set. The CUBIC clearing protocol can process multiple samples simultaneously. We previously used CUBIC to image whole-brain neural activities at single-cell resolution using Arc-dVenus transgenic (Tg) mice. CUBIC informatics calculated the Venus signal subtraction, comparing different brains at a whole-organ scale. These protocols provide a platform for organism-level systems biology by comprehensively detecting cells in a whole organ or body.
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|Item Type:||Journal Article|
|Divisions:||Current > Schools > School of Electrical Engineering & Computer Science
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2015 Nature Publishing Group|
|Deposited On:||11 Oct 2015 23:04|
|Last Modified:||02 May 2016 03:40|
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