Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging

Susaki, Etsuo A, Tainaka, Kazuki, Perrin, Dimitri, Yukinaga, Hiroko, Kuno, Akihiro, & Ueda, Hiroki R (2015) Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging. Nature Protocols, 10(11), pp. 1709-1727.

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Abstract

Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples. The organ or body is cleared by immersion for 1–14 d, with the exact time required dependent on the sample type and the experimental purposes. A single imaging set can be completed in 30–60 min. Image processing and analysis can take <1 d, but it is dependent on the number of samples in the data set. The CUBIC clearing protocol can process multiple samples simultaneously. We previously used CUBIC to image whole-brain neural activities at single-cell resolution using Arc-dVenus transgenic (Tg) mice. CUBIC informatics calculated the Venus signal subtraction, comparing different brains at a whole-organ scale. These protocols provide a platform for organism-level systems biology by comprehensively detecting cells in a whole organ or body.

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19 citations in Web of Science®

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ID Code: 88909
Item Type: Journal Article
Refereed: Yes
DOI: 10.1038/nprot.2015.085
ISSN: 1750-2799
Divisions: Current > Schools > School of Electrical Engineering & Computer Science
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2015 Nature Publishing Group
Deposited On: 11 Oct 2015 23:04
Last Modified: 02 May 2016 03:40

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