An N-ethyl-n-nitrosourea induced corticotropin-releasing hormone promoter mutation provides a mouse model for endogenous glucocorticoid excess

Bentley, Liz, Esapa, Christopher T., Nesbit, M. Andrew, Head, Rosie A., Evans, Holly, Lath, Darren, Scudamore, Cheryl L, Hough, Tertius A., Podrini, Christine, Hannan, Fadil M., Fraser, William D., Croucher, Peter I., Brown, Matthew A., Brown, Steve D.M., Cox, Roger D., & Thakker, Rajesh V. (2014) An N-ethyl-n-nitrosourea induced corticotropin-releasing hormone promoter mutation provides a mouse model for endogenous glucocorticoid excess. Endocrinology, 155(3), pp. 908-922.

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Cushing's syndrome, which is characterized by excessive circulating glucocorticoid concentrations, maybe due to ACTH-dependent or -independent causes that include anterior pituitary and adrenal cortical tumors, respectively. ACTH secretion is stimulated by CRH, and we report a mouse model for Cushing's syndrome due to an N-ethyl-N-nitrosourea (ENU) induced Crh mutation at -120 bp of the promoter region, which significantly increased luciferase reporter activity and was thus a gain-of-function mutation. Crh -120/+ mice, when compared with wild-type littermates, had obesity, muscle wasting, thin skin, hair loss, and elevated plasma and urinary concentrations of corticosterone. In addition, Crh-120/+ mice had hyperglycemia, hyperfructosaminemia, hyperinsulinemia, hypercholesterolemia, hypertriglyceridemia, and hyperleptinemia but normal adiponectin. Crh -120/+ mice also had low bone mineral density, hypercalcemia, hypercalciuria, and decreased concentrations of plasma PTH and osteocalcin. Bone histomorphometry revealed Crh-120/+ mice to have significant reductions in mineralizing surface area, mineral apposition, bone formation rates, osteoblast number, and the percentage of corticoendosteal bone covered by osteoblasts, which was accompanied by an increase in adipocytes in the bone marrow. Thus, a mouse model for Cushing's syndrome has been established, and this will help in further elucidating the pathophysiological effects of glucocorticoid excess and in evaluating treatments for corticosteroid-induced osteoporosis.

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ID Code: 89078
Item Type: Journal Article
Refereed: Yes
Keywords: adiponectin, corticosterone, corticotropin releasing factor, ethylnitrosourea, fructosamine, glucocorticoid, luciferase, mineral, osteocalcin, parathyroid hormone, adipocyte, animal experiment, animal model, article, base pairing, bone density, bone mineralization, controlled study, corticosterone blood level, Cushing syndrome, enzyme activity, experimental obesity, female, gain of function mutation, hair loss, hypercalcemia, hypercalciuria, hypercholesterolemia, hyperglycemia, hyperinsulinemia, hyperleptinemia, hypertriglyceridemia, kidney concentrating capacity, male, mouse, muscle atrophy, nonhuman, ossification, osteoblast, osteometry, parathyroid hormone blood level, priority journal, promoter region, skin disease, wild type, Animals, Body Composition, Bone and Bones, Calcium, Cell Line, Chromosome Mapping, Corticotropin-Releasing Hormone, Disease Models, Animal, Glucocorticoids, Lipid Metabolism, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Mutation, Osteoblasts, Osteoporosis, Promoter Regions, Genetic
DOI: 10.1210/en.2013-1247
ISSN: 0013-7227
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 15 Oct 2015 23:22
Last Modified: 17 Feb 2016 04:19

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