A technique for the measurement of renal ATP in a large animal model of septic shock
May, Clive, Wan, Li, Williams, John, Wellard, R. Mark, Pell, Gaby S., Bellomo, Rinaldo A., & Jackson, Graeme D. (2005) A technique for the measurement of renal ATP in a large animal model of septic shock. International Journal of Artificial Organs, 28(1), pp. 16-21.
BACKGROUND: The mechanisms responsible for acute renal failure in sepsis are not understood. Measurement of tissue ATP might help to understand this process but, in the large animal, it is hampered by major technical difficulties. OBJECTIVE: To develop a technique to monitor ATP in the kidney of a large mammal during the induction of septic shock and then circulatory arrest. METHODS: Implantation of a custom-made phosphorus coil around the left kidney. Induction of septic shock by intravenous E. coli administration. Acquisition of 31 P magnetic resonance (MR) spectroscopic data at 3-tesla before and during septic shock over several hours. Induction of euthanasia and measurement of the same 31 P signal immediately and thirty minutes after circulatory arrest. RESULTS: Clear reproducible 31 P MR spectra were obtained before and after the induction of septic shock and euthanasia. They indicated limited changes in ATP during septic shock. An expected rapid and dramatic decrease in ATP occurred with euthanasia. CONCLUSIONS: It is possible to sequentially monitor renal bioenergetics in a large mammal during septic shock using an implanted custom-made phosphorus coil and 3-tesla MR technology. This technique offers a novel approach to the investigation of septic renal failure.
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|Item Type:||Journal Article|
|Additional Information:||For more information, please refer to the journal’s website (see hypertext link) or contact the author. Author contact details: firstname.lastname@example.org|
|Keywords:||Kidney, ATP, Sepsis, Acute renal failure, Shock, Magnetic resonance spectroscopy|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Research Centres > Science Research Centre
|Copyright Owner:||Copyright 2005 Wichtig Editore|
|Deposited On:||08 Aug 2007|
|Last Modified:||29 Feb 2012 23:20|
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